The Phage Cookbook by Dominik Refardt
Glycerol/NaOH: 150 ml water, 4.25 g NaCl, add Glycerol to 500 mL, autoclave, aliquot in 50 mL tubes and store at -20 °C
Mix 5 parts bacterial culture with 4 parts Glycerol/NaOH
Mix by pipetting up and down and store at -80 °C
Or: Prepare 50% glycerol, autoclave. Mix 3:7 with bacterial culture (= 15% glycerol).
Storing the glycerol in aliquots in the freezer prevents contamination and ensures peace of mind when using it in irregular intervals.
Phage lysates (in SMG) can be kept at 4 °C. Add a drop of chloroform to prevent fungal or bacterial growth. The chloroform has to be replaced from time to time because it evaporates.
If storage at -80 °C is required, use the recipes below.
Add 35 µL DMSO to a cryotube
Dilute 50 µL phage lysate in 500 µL LB and add
Mix by pipetting up and down and store at -80 °C
Alternatively, phage lysates can be frozen in 50% glycerol (not 15%, like it is used with bacteria!)