The Phage Cookbook by Dominik Refardt
Stock concentration: 100 mg/mL in water (filter sterilize), store at -20 °C.
Working concentration: 100 µg/mL
Toxic! Work under the hood!
Bacteriostatic, binds to the 50S ribosome and inhibits peptidyl transferase activity.
Stock concentration: 15 mg/mL in 100% EtOH (no need to filter sterilize), protect from light, store at -20 °C.
Working concentration: 15 µg/mL
Bactericidal, 30S inhibitor (aminoglycoside)
Stock concentration: 50 mg/mL in water (filter sterilize), store at -20 °C.
Working concentration: 50 µg/mL
Quinolone. Binds to the A subunit of DNA gyrase (topoisomerase) and prevents supercoiling of DNA, thereby inhibiting DNA synthesis.
Stock concentration: 30 mg/mL in 300 mM NaOH
Working concentration: 30 µg/mL
10 µL Stock concentration: solution diluted in 50 mL and spread on a plate inhibits growth. 1 µl Stock concentration: solution diluted in 50 mL and spread on a plate does not inhibit growth.
Bactericidal, 30S inhibitor (aminoglycoside)
Stock concentration: 100 mg/mL in water (filter sterilize), store at -20 °C.
Working concentration: 50 µg/mL
Bacteriostatic, 30S inhibitor (tetracycline)
Stock concentration: 10 mg/mL in 50% EtOH (filter sterilize), protect from light, store at -20 °C.
Working concentration: 15 µg/mL