Phage Cookbook

The Phage Cookbook

Here you find my collection of notes about how to handle phage λ and other phages. I give no guarantee that any of this works - for me it does though. If you spot an error, please tell me.

Table of Contents

Recipes / Handling chemicals

• Media and buffers

• Antibiotics

• Various chemicals

• Enzymes

Various Methods

• DNA isolation from λ lysates using the Wizard^® DNA Clean-Up System (Promega)

• DNA isolation from λ lysates using the Qiagen lambda midi kit

• Confirming single copy λ prophage integration (Powell et al. 1994)

• Recombineering/λ red-mediated gene replacement

• P1 Transduction

• DNA Sequencing

• TSS Transformation

• Flow Cytometry

Handling phages

• Preparing a phage stock

• How to store bacteria and phage

• Quantification of phages - on a plate

• Quantification of phages - real time PCR with SYBR Green

• How to make a proper dilution series

• Using λ JL259, which makes blue colonies

Collections

• List of all strains in the collection

• List of all PCR primers

Other things

• References

• Expressing yourself properly