Dr Ellen Allwood
Contact
Email: e.allwood(theatsign)sheffield.ac.uk
Lab: D59
Office: C34
Career/ Education
Post-doctoral Research Assistant (June 2021-present). School of Biosciences, University of Sheffield. “A plant-specific motor powering cell division and seed development”. Funded by The Leverhulme Trust.
Post-doctoral Research Assistant and Lab manager (2005-2022). Molecular Biology and Biochemistry/Biomedical Science, University of Sheffield. "Regulation of yeast endocytosis through the actin cytoskeleton" (Prof Kathryn Ayscough).
Post-doctoral Research Assistant (1998-2005). University of London (Royal Holloway and Bedford New College), and University of Durham. "Phosphorylation and regulation of actin binding proteins in plants" (Prof Patrick Hussey).
Post-doctoral Research Assistant (1996-1998). University of London (Royal Holloway and Bedford New College). "Phosphorylation of plant Phenylalanine ammonia-lyase in plant cell walls". (Prof. Paul Bolwell and Dr Dewi Davies)
D.Phil. Plant primary metabolism (1992-1996) St Hilda's College, Oxford. PhD in plant primary metabolism. "Control of 6-phosphofructo-2-kinase/2,6-bisphosphatase in plants". (Supervisor Dr Nick Kruger)
Research Assistant (1991-1992). University of Oxford (Plant Sciences). "Kinetic analysis of plant primary metabolic enzymes". (Dr Nick Kruger).
B.A.hons FHSNS Botany (1991), St Hilda's College, Oxford.
Publications
Phosphorylation of the WH2 domain in yeast Las17/ WASP regulates G actin binding and protein function during endocytosis J. J. Tyler; I. Smaczynska de Rooij; L. Abugharsa; J. S. Palmer; L. P. Hancock; E. G. Allwood; & K. R. Ayscough. Scientific reports 11 (9718) 2021.
The Pex3–Inp1 complex tethers yeast peroxisomes to the plasma membrane. GE Hulmes, JD Hutchinson, N Dahan, JM Nuttall, EG Allwood, KR Ayscough, EH Hettema Journal of Cell Biology 219 (10) 2020
Mutation of key lysine residues in the Insert B region of the yeast dynamin Vps1 disrupts lipid binding and causes defects in endocytosis. Smaczynska-de Rooij, Iwona I; Marklew, Christopher J; Palmer, Sarah E; Allwood, Ellen G; Ayscough, Kathryn R. PloS one 14 (4) 2019
An immune-responsive cytoskeletal-plasma membrane feedback loop in plants Stefan Sassmann, Cecilia Rodrigues, Stephen W Milne, Anja Nenninger, Ellen Allwood, George R Littlejohn, Nicholas J Talbot, Christian Soeller, Brendan Davies, Patrick J Hussey, Michael J Deeks. Current Biology 28 (13) 2136-2144. 2018.
Elucidating Key Motifs Required for Arp2/3-Dependent and Independent Actin Nucleation by Las17/WASP. EG Allwood, JJ Tyler, AN Urbanek, II Smaczynska-de Rooij, KR Ayscough Plos one 11 (9), e0163177 2016
Phosphorylation regulates the endocytic function of the yeast dynamin-related protein Vps1 Iwona I Smaczynska-de Rooij, Christopher J Marklew, Ellen G Allwood, Sarah E Palmer, Wesley I Booth, Ritu Mishra, Martin W Goldberg, Kathryn R Ayscough 2016 Molecular and cellular biology 36 (5) 742-755 2016
WASP family proteins, more than Arp2/3 activators JJ Tyler, EG Allwood, KR Ayscough Biochemical Society Transactions 44 (5), 1339-1345 2016
A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast SE Palmer, II Smaczynska-de Rooij, CJ Marklew, EG Allwood, R Mishra, Simeon Johnson, Martin W Goldberg, Kathryn R Ayscough Current Biology 25 (7), 868-878 2015
Distinct actin and lipid binding sites in Ysc84 are required during early stages of yeast endocytosis. Urbanek, Agnieszka N; Allwood, Ellen G; Smith, Adam P; Booth, Wesley I; Ayscough, Kathryn R. PloS one 10 (8) 2015.
A Charge Swap mutation E461K in the yeast dynamin Vps1 reduces endocytic invagination. Palmer, Sarah E; Smaczynska-de Rooij, Iwona I; Marklew, Christopher J; Allwood, Ellen G; Mishra, Ritu; Goldberg, Martin W; Ayscough, Kathryn R. Communicative & integrative biology. 8 (4) 2015.
Yeast endocytic adaptor AP-2 binds the stress sensor Mid2 and functions in polarized cell responses. Chapa-y-Lazo, Bernardo; Allwood, Ellen G; Smaczynska-de Rooij, Iwona I; Snape, Mary L; Ayscough, Kathryn R. Traffic 15 (5) 2014.
A Novel Actin-Binding Motif in Las17/WASP Nucleates Actin Filaments Independently of Arp2/3 AN Urbanek, AP Smith, EG Allwood, WI Booth, KR Ayscough Current Biology 2013
Yeast Dynamin Vps 1 and Amphiphysin Rvs 167 Function Together During Endocytosis Smaczynska-de Rooij, Iwona I; Allwood, Ellen G; Mishra, Ritu; Booth, Wesley I; Aghamohammadzadeh, Soheil; Goldberg, Martin W; Ayscough, Kathryn R. Traffic. 13 (2) 2012.
A role for the dynamin-like protein Vps1 during endocytosis in yeast. Iwona I Smaczynska-de Rooij, Ellen G Allwood, Soheil Aghamohammadzadeh, Ewald H Hettema, Martin W Goldberg, Kathryn R Ayscough. Journal of cell science 123 (20) 3496-3506. 2010
The WASP homologue Las17 activates the novel actin-regulatory activity of Ysc84 to promote endocytosis in yeast. Robertson, Alastair S; Allwood, Ellen G; Smith, Adam PC; Gardiner, Fiona C; Costa, Rosaria; Winder, Steve J; Ayscough, Kathryn R. Molecular biology of the cell 20 (6) 2009.
Interactions between the yeast SM22 homologue Scp1 and actin demonstrate the importance of actin bundling in endocytosis. Gheorghe, Dana M; Aghamohammadzadeh, Soheil; Smaczynska-de Rooij, Iwona I; Allwood, Ellen G; Winder, Steve J; Ayscough, Kathryn R. Journal of Biological Chemistry 283 (23) 2008
Actin organization and root hair development are disrupted by ethanol-induced overexpression of Arabidopsis actin interacting protein 1 (AIP1) T Ketelaar*, EG Allwood*, PJ Hussey New Phytologist 174 (1), 57-62 2007
Oscillatory increases in alkalinity anticipate growth and may regulate actin dynamics in pollen tubes of lily. Lovy-Wheeler, Alenka; Kunkel, Joseph G; Allwood, Ellen G; Hussey, Patrick J; Hepler, Peter K. The Plant Cell 18 (9) 2006
The actin-interacting protein AIP1 is essential for actin organization and plant development T Ketelaar, EG Allwood, R Anthony, B Voigt, D Menzel, PJ Hussey Current Biology 14 (2), 145-149 2004
Regulation of CDPKs, including identification of PAL kinase, in biotically stressed cells of French bean. EG Allwood, DR Davies, C Gerrish, GP Bolwell. Plant molecular biology 49 (5), 533-544 2002.
Regulation of the pollen-specific actin-depolymerizing factor LlADF1 EG Allwood, RG Anthony, AP Smertenko, S Reichelt, BK Drobak, John H Doonan, Alan G Weeds, Patrick J Hussey. The Plant Cell 14 (11), 2915-2927 2002
Actin-binding proteins in the Arabidopsis genome database: properties of functionally distinct plant actin-depolymerizing factors/cofilins Hussey, Patrick J; Allwood, Ellen G; Smertenko, Andrei P. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 357 (1422) 2002
Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase EG Allwood, AP Smertenko, PJ Hussey FEBS letters 499 (1-2), 97-100 2001
Interaction of pollen‐specific actin‐depolymerizing factor with actin. Andrei P Smertenko, Ellen G Allwood, Safina Khan, Chang‐Jie Jiang, Sutherland K Maciver, Alan G Weeds, Patrick J Hussey The Plant Journal 25, 203-212. 2001
Phosphorylation of phenylalanine ammonia-lyase: evidence for a novel protein kinase and identification of the phosphorylated residue. EG Allwood, DR Davies, C Gerrish, BE Ellis, GP Bolwell Febs Letters 457 (1), 47-52 1999
Inhibition of pyrophosphate: fructose-6-phosphate 1-phosphotransferase by imidodiphosphate. Rowntree, Ellen G; Kruger, Nicholas J. Plant Science 86 (2) 1992.