Immunofluorescence localization of alpha-actin and myosin in c2c12 cells. The days of fixation include prior to differentiation (A) at myoblast stage (MB), Day 0 (B), Day 5 (C), Day 7 (D), and negative control Day 12 (E). Three sets of data include 1) myosin (green) and alpha-actin (red). Alpha-actin is expressed throughout myoblast (MB) until Day 7, indicating that a form of alpha-actin is present within the differentiation of c2c12 cells.

Alpha-actin is localized within the sampling days from MB to Day 7.