The variation in culture conditions was done to observe the range of potential effects of different serums and confluency during the process of myogenesis. This target subgroup is focusing on how the lack of growth factors in 10% Horse Serum (HS) starvation media (SM) affects the growth and differentiation of the C2C12 cells compared to its control counterparts which were grown exclusively in 10% fetal bovine serum (FBS). To examine the impact of serum, C2C12 cells are first allowed to reach 100% confluency (Day 0) in 10% FBS, then swapped with 10% HS SM for the rest of the experiment or alternatively maintained on 10% FBS.

As such, our research question is:

How does initiating differentiation with 10% HS at 100% confluency impact C2C12 cells during myogenesis, compared to the C2C12 cells maintained in 10% FBS?

Antibodies used for immunostaining had non-specific binding, as such no conclusion about myoD and m-Cadherin localization can be made.

MyoD and m-Cadherin expression in both control and experimental groups exhibit the same expression trend.

Expression of myoD in the experimental group is lower than in the control group. The m-Cadherin expression had no statistical difference between either culture condition.