Continue to produce lectin staining and AB/PAS images to quantify mucus thickness and goblet cell size
Optimization of FISH + lectin fluorescent staining procedure to characterize spatial arrangements of the gut microbiome
Correlation of Islet images to FISH images to see what the gut microbiome looks like at varying stages of insulitis
Use flow cytometry to characterize immune cell population
We found that Methanol Carnoy's fixative prevents insulin antibody binding
Mucus agglutinin staining is more effective than the MUC2 antibody for detecting the mucus layer