Embedded Files
Tomato Lectin staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of cortex (left) and cortex (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Blocking for 2 days in Antibody Blocking Solution at 37C.
Radiant Labeling in SmartBatch+ using 80 µL Lectin-594 in the Single Sample Staining Cup.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
Page updated
Report abuse