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Thioflavin S staining in a whole mouse brain from a 5XFAD mouse crossed with a BXD29 mouse (40 µm max intensity projection). Sample provided by David Ashbrook and Robert W. Williams, University of Tennessee Health Science Center, and G. Allan Johnson, Duke Center for In Vivo Microscopy.
Zoom in of hippocampus (left) and cortex/striatum (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Radiant Labeling in SmartBatch+ using 24 µg Thioflavin S in the Single Sample Staining Cup.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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