Embedded Files
Rat SST staining in a whole mouse brain (40 µm max intensity projection). Click the button to view the 3D dataset in Neuroglancer.
View 3D Dataset
Zoom in of hippocampus (left) and cortex (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 6 µg Rat anti-SST antibody in the Single Sample Staining Cup.
Secondary Labeling with 12 µg Donkey anti-Rat SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
Page updated
Report abuse