Embedded Files
Rat GFAP staining in a whole mouse brain (40 µm max intensity projection). Click the button to view the 3D dataset in Neuroglancer.
Zoom in of hippocampus (left) and cerebellum (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 10 µg Rat anti-GFAP antibody in the Single Sample Staining Cup.
Secondary Labeling with 20 µg Donkey anti-Rat SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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