Embedded Files
Rabbit Transthyretin staining in a whole E12.5 embryonic mouse brain (40 µm max intensity projection) in cyan. Sample provided courtesy of Eboni Monae Arnold in the Lehtinen Lab at Boston Children's Hospital.
Zoom in of choroid plexus below.
Protocol
Protocol
SHIELD fixation following the Organoid Protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Radiant Labeling in SmartBatch+ using 1 µg Rabbit anti-TTR in the Single Sample Staining Cup.
Secondary Labeling with 2 µg Donkey anti-Rabbit SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
Page updated
Report abuse