Embedded Files
Rabbit PKC Delta staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of thalamus(left) and cortex(right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 4 µg Rabbit Anti-PKC Delta antibody in the Single Sample Staining Cup.
Secondary Labeling with 8 µg Donkey anti-Rabbit SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
Page updated
Report abuse