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Rabbit NPY staining in a whole mouse brain (40 µm max intensity projection). Click the button to view the 3D dataset in Neuroglancer.
Zoom in of hippocampus (left) and cortex (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 6 µg Rabbit Anti-NPY antibody in the Single Sample Staining Cup.
Secondary Labeling with 12 µg Donkey anti-Rabbit Alexa 555+ in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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