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Rabbit Melanin concentrating hormone staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of hypothalamus/thalamus (left) and hindbrain (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 6 µg Rabbit Anti-MCH antibody in the Single Sample Staining Cup.
Secondary Labeling with 12 µg Donkey anti-Rabbit Alexa 555+ in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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