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GLP-1R staining in a whole mouse brain (transverse, 40 µm max intensity projection).
Zoom in of the paraventricular hypothalamic nucleus (PVH).
Zoom in of the lateral hypothalamic area (LHA).
Zoom in of the nucleus accumbens (NAc).
Zoom in of the striatum (STR).
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Radiant Labeling in SmartBatch+ using 4 µg Rabbit anti-GLP-1R antibody in the Single Sample Staining Cup.
Secondary Labeling with 6 µg Donkey anti-rabbit SeTau647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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