Embedded Files
Rabbit c-Fos staining in a whole rat brain (40 µm max intensity projection). Click the button to view 3D dataset in Neuroglancer.
Zoom in of hippocampus (left) and cortex (right) below.
Zoom in of striatum (left) and thalamus (right) below.
Protocol
Protocol
Standard SHIELD protocol for rat brain (6 days SHIELD OFF, 1 day SHIELD ON).
Active delipidation in SmartBatch+ for 6 days.
4 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 17.5 µg Rabbit Anti-cFos antibody in the Medium Staining Cup.
Secondary Labeling with 26.25 µg Donkey anti-Rabbit SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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