Embedded Files
Rabbit Aggrecan staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of cortex (left) and hippocampus (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Radiant Labeling in SmartBatch+ using 6 µg Rabbit Anti-Aggrecan antibody in the Single Sample Staining Cup.
Secondary Labeling with 12 µg Donkey anti-Rabbit SeTau-647 in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
Page updated
Report abuse