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Mouse Neurophysin 1 staining in a whole mouse brain (40 µm max intensity projection).
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Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
2 days blocking with Blocking Buffer at 37°C.
Radiant Labeling in SmartBatch+ using 4 µg Mouse anti-Neurophysin 1 antibody in the Single Sample Staining Cup.
Secondary Labeling with 8 µg Donkey anti-Mouse Alexa 555+ in SmartBatch+.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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