Goat Olig2 (R&D Systems AF2148)

Goat Olig2 staining in a whole mouse brain (40 µm max intensity projection).

Zoom in of hippocampus (left) and cerebellum (right) below. 

Protocol

1.  Standard SHIELD protocol.

2.  Passive delipidation with Clear+ Delipidation Buffer.

3.  2 days blocking with Blocking Buffer at 37°C.

4.  Radiant Labeling in SmartBatch+ using 6 µg Goat Anti-Olig2 antibody in the Single Sample Staining Cup. 

5.  Secondary Labeling with 12 µg Donkey anti-Goat SeTau-647 in SmartBatch+.

6.  EasyIndex index matching and mounting. 

7. Imaged on SmartSPIM with 3.6X objective.