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Alpaca GFAP staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of cortex (left) and hippocampus (right) below.
Protocol
Protocol
Standard SHIELD protocol.
Passive delipidation with Clear+ Delipidation Buffer.
Radiant Labeling in SmartBatch+ using 1 µg Alpaca anti-GFAP nanobody in the Single Sample Staining Cup.
EasyIndex index matching and mounting.
Imaged on SmartSPIM with 3.6X objective.
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