TIRE

About the Total Internal Reflection Ellipsometry (TIRE)

• Why did we select TIRE?

• Surface Plasmon Resonance (SPR)

• Total Internal Reflection Ellipsometry

• (Reflectance related parameter) ψ + (Changed phase of light related parameter) Δ

• Useful to obtain the optical properties and physical quantities (layer thickness and multi layer morphology)

• Our experimental setup

Study Case 1. Optical characteristic of proteins

Change of TIRE Spectrum as Adsorption of Protein

Optical Model Calculation of Protein

Study Case 2. Total Internal Reflection Imaging Ellipsometry (TIRIE)

Schemetic of Imaging Ellipsometry

Protein chip using TIRIE

Change of TIRIE Image as Adsorption of Protein

• Summery

• obtained layer thickness, complex refractive index of bio-molecules (BSA, SA, HSA, oligo-DNA), which are in buffer.

• report the spectroscopic optical property of bio-molecules (SA, HSA, oligo-DNA) for the first time.

• can confirm the kinetics of interaction between bio-molecules (BSA and SA; HSA and oligo-DNA) using TIRE.

• be used as high sensitive in-situ monitoring for the study of interaction of bio-molecules.

• can be used as multi array sensors for detection of bio-molecules

• Appendix : Evanescent wave