My Internship

Dr. Marimar Hernandez-Perez (left) and Naomi Riley (right)

My Internship Experience

During the 2019-2020 academic year I've had the opportunity to work in the R4 Research building in the Diabetes Research Lab under Dr. Marimar Hernandez-Perez. The lab focused on the development of the pancreas and the migration of macrophages in zebrafish and mice to determine possible drugs and treatments that can be used to treat diabetes. This research is significant due to the 400,000 people in the United States alone that are diagnosed with diabetes every year. This part of the study will allow us to apply the research to the pancreas in order to determine if the same technique can be applied to damaged beta cells to therefore determine if the signaling of the macrophages can be altered. Ultimately allowing the beta cells of the pancreas to heal themselves and produce a once again functioning pancreas.

*Poster only available for view on 4/29*

Internship Summary

Immune response to inflammatory signals is a major contributor to Type1 and Type2 diabetes. Understanding macrophage infiltration to pancreatic islets that leads to beta cell death is of great importance to develop therapies for diabetes. Immune cell infiltration into the pancreas is, in part, produced by the proinflammatory signals synthesized by the enzyme 12-lipooxygenase (12-LOX). We developed an investigational therapy with a 12-LOX inhibitor (ML355) that reduces diabetes development in mice. In this project we look at how 12-LOX affects macrophage migration to injuries. To establish a model for studying migration of proinflammatory macrophages we used a transgenic line of zebrafish engineered to express the green fluorescent protein (GFP) wherever the pro-inflammatory cytokine TNFa is expressed, facilitating the visualization of macrophage. As for the injury model, we performed tail cuts to TNFa-GFP zebrafish embryos and quantify the amount of TNFa+ cells at the injury site. To study the role of 12-LOX in migration we treated the embryos with ML355. After 6 hours, embryos are collected and analyzed using confocal microscopy. Conclusions from this work will help determine if the signaling of immune cells to damaged beta cells can be modulated preventing damage and the subsequent development of diabetes.

My Role

My lab duties within the lab consisted of breeding the zebrafish, collecting the eggs, preparing petri dishes that contained viable embryos, making the desired amount of antibody needed to performing washes on the embryos, creating the needles that would later be utilized to perform the frug injections, perform the tail assays and finally mounting the embryos on slides and imaging the slides on a confocal microscope. The work that I performed was very important to the success of the project, if any mistakes were made it would potentially set back the progress of the project and ultimately skew the results of the project. Due to circumstances brought on by COVID-19 I was not able to complete the final portion of the project and analyze the data, therefore the data was analyzed by my supervisor.

Zebrafish Egg Development

This video provides more information as to how zebrafish embryos develop over a period of 24 hours.

Breeding Zebrafish

This video serves as visual detail to show how zebrafish are breed, eggs are collected and injected with morpholino for further research analysis.

Zebrafish Microinjection

This video shows in detail the process of microinjecting the morpholino into the zebrafish embryos.

Why Did I Choose this Internship?

I choose this internship because of my family's history with diabetes. My mom has diabetes and so do a couple of my other family members and I have seen my mom struggle with the disease over the years. I don't believe that the treatment options that she has been given are the best for her. I think the work that the lab is doing is so important and being able to say that I took part in something like this is amazing.

How this Internship will Allow me to Succeed

I believe that this internship allowed me to succeed in my understanding of lab work. I was able to understand the intricacies that go into completing a research project and all of the time that it takes in order to get the desired data. I also gained more in depth lab skills and also learn technical skills about how to properly utilize the microscopes, how to make needles within the lab, and also the importance of washing the embryos within the experiment. The skills that I have learned from the lab can be used my current biology and chemistry lab courses and the skills can potentially be utilized in my future career.

My Ideal Workplace

During my time at my internship I was able to personally observe a lot of the work was completed independently, but the work contributed to the overall success of the project. There were not often tasks that were completed collectively, the majority of the work was completed independently.However there were often meetings held to check on the personal and overall progress of the project and to provide feedback My supervisor made sure that I was properly trained on how to work the equipment, collect the zebrafish embryos, image the data and properly format the poster. The environment of my internship is very helping and hands-on when it comes to any questions that I have. I knew that if I ever had a question about a task that I was assigned or if a mistake was made I knew that I could ask for help and guidance on the correct way to do things would be provided.

I would describe my ideal workplace as a friendly and energetic environment where I am constantly learning new things and interacting with new people. Also a place where I am able to develop and grow professionally and financially. I enjoyed that my internship site was an open place for questions which allowed me to learn without fear of looking bad in front of my supervisor for not knowing all the answers. I definitely want to make sure that my future workplace is the same, in that people within the workplace are not afraid to ask questions or ask for help because it will be a understanding and compassionate environment.

Growth As a Professional

After spending a few months observing the professionals at my site I have been able to observe some of the professional mannerisms that have been conducted within the lab. For example when there are important meetings that my supervisor needs to go to she will make sure to notify someone else within the lab so that the tasks can still be completed or she will try to complete them from home. When she is not able to be present within the lab during the times that I am scheduled in she will make sure to provide me with instructions on the things that need to be done and also make sure I am able to reach her if I have any issues. Being in my internship environment has also allowed me to experience how important flexibility and communication is in order to be a researcher within the lab.