Embedded Files
Use of imaging software to measure intensity
Activities Involved
Activities Involved
Digestive gland sample of around half an inch was used
Sections of 8 µm thickness taken using a cryostat
The sections were stained using lipofuscin
The stained slides were mounted using Eukit (Xylene)
The mounted slides were observed under a microscope and snapshots were taken using an imaging software
The snapshots were then used to measure intensity of granules in the sample
Results
Results
The intensity of the granules was recorded in an excel file and the graph on the right was created. The control provides us with a baseline for comparison.
Increased levels of lipofuscin indicate oxidative stress in the organism. The reproductive season for mytilus must also be accounted for as the mussels undergo higher levels of stress during reproductive development and gamete production.
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