Protein Content by lowry method
Homogenization of samples with K-phosphate buffer
Testing of reaction with hydrogen peroxide with a spectrophotometer
Testing of protein content using the Lowry method
Catalase is a common enzyme found in all aerobic organisms, It catalyses the reaction converting hydrogen peroxide to water and oxygen. The analysis uses hydrogen peroxide to test the amount of catalase in the cell. The higher the catalase more the oxidative stress, experienced by the organism, the faster the reaction occurs. It can be noticed that the catalase activity is increased in March 2021 due to gonad development.