Senior high science requires heightened awareness, specialized equipment, and stricter controls. Teachers must model professional laboratory standards and students must develop deep respect for procedures and protocols.
General High School Science Lab Safety Considerations
Personal Conduct and Preparedness
Follow all teacher instructions before, during, and after the lab activity.
Do not begin any experiment until told to do so.
Read procedures in advance and ask questions if unsure.
Stay at your assigned station unless directed otherwise.
Report any injury, spill, or equipment issue immediately.
Personal Protective Equipment (PPE)
Wear safety goggles when working with heat, chemicals, glassware, or projectiles.
Tie back long hair and secure loose clothing or jewelry.
Wear closed-toe shoes—no sandals or bare feet.
Lab coats are recommended, especially for Chemistry labs.
Wear gloves when handling chemicals, preserved specimens, or microorganisms, as instructed.
General Hygiene and Safety Rules
Wash your hands after each lab, especially before eating or touching your face.
Never eat or drink in the lab, and never use lab glassware as food or drink containers.
Minimize, when possible, personal items such as backpacks and coats in lab work areas—store them safely away.
Electrical and Equipment Safety
Keep water away from electrical equipment.
Use only dry hands when operating electrical devices.
Inspect for frayed wires or damaged plugs—report issues immediately.
Turn off equipment when not in use.
Heat and Flame Safety
Never leave open flames unattended.
Use caution with hot plates, burners, or heated glassware.
Treat all objects as potentially hot unless confirmed otherwise.
Keep combustibles and flammables away from ignition sources.
Handling Substances and Equipment
Waft to smell—never directly inhale chemicals.
Never taste any substance in the lab.
Only use materials and equipment as instructed by your teacher.
Dispose of materials properly—never pour chemicals back into stock bottles or down the sink without approval.
Emergency Awareness
Know the location of:
Eyewash station
Safety shower
Fire extinguisher
Fire blanket
Emergency exits and evacuation routes
Participate seriously in safety drills and emergency walkthroughs.
Biology Lab Hazards and Safety Considerations
Permitted:
Dissection of organs purchased from certified scientific suppliers
Safe use of bacteria (non-pathogenic), fungi, and protists
Observation of plant cells, invertebrates, and aquatic organisms
Prohibited:
Mammalian blood or tissues (except federally inspected specimens)
Cheek cell scrapings, urine, feces, or human tissue
Culturing swabs from human sources or soil (to avoid pathogens)
Safety Measures:
Always use disposable gloves, disinfect surfaces, and wash hands thoroughly.
Petri dishes must be taped shut and sterilized after use.
Never allow food in labs that involve biological samples.
Chemistry Lab Hazards and Safety Considerations
Permitted (with safeguards):
Acids and bases, including HCl and NaOH
Common salts, indicators (phenolphthalein, bromothymol blue), and solvents (ethanol, acetone)
Safe oxidizers and reducers in small, diluted quantities
Hydrogen gas, ammonia, carbon dioxide – with fume hood use
Restricted Use:
Use of nitric acid is permitted only with proper storage, fume hoods, and teacher expertise.
Prohibited:
All substances listed in the Prohibited Chemical List
Mercury and any of its compounds
Explosives, peroxides, and most cyanides
Chemical Storage and Lab Design
High school labs must have:
Separate locked cabinets for acids, flammables, and poisons
Vented storage for volatile chemicals
Wooden shelves firmly secured to the wall (no metal clips)
Colour-coded labels and MSDS access for all substances
No alphabetical storage (to prevent incompatible pairings)
Chemical quantities should be kept to a minimum, and an updated inventory should be available for the fire department.
Emergency Equipment and Procedures
Senior labs must be equipped with:
ABC fire extinguisher
Clearly labelled gas shut-off valve
Fire blanket, eyewash station, and first-aid kit
Emergency shower (within 15 m of all stations)
Fume hood with airflow ≥ 0.5 m/s, not used for storage
Spill kits for acids, bases, and organic solvents
Regular equipment inspections and fire drills are required.
Physics Lab Hazards and Safety Considerations
Electrical Safety
Electricity is a core focus in physics and carries serious risk if misused.
Core Guidelines:
Use equipment at rated capacity only
Always turn off equipment when not in use
Insulate all live parts of circuits
Clearly label high vs. low voltage connections (e.g., induction cells)
Risk Reduction Measures:
Use fuses or circuit breakers to limit amperage
Dry hands only when working with circuits
Avoid working near water or wet benches
Stand on rubber mats or dry carpet
Use only CSA-approved equipment; check regularly for frayed wires or leakage
Specific Hazards:
Never bridge a live circuit with both hands (risk of electric shock)
Secure cords, and don’t run them across walkways
Mechanical Hazards
Rotating or vibrating devices used in physics (e.g., motors, gyroscopes) pose entanglement and pinch hazards.
Ensure all moving parts are guarded
Tie back long hair and avoid loose clothing
Always wear eye protection when working with rotating equipment
Periodically inspect devices for wear and tear
Radiation and Light Hazards
Physics labs may include lasers, light sources, and radioactive samples.
Visible Light:
Never look directly at bright light sources (e.g., burning magnesium)
Strobe lights may cause seizures in susceptible students
Lasers:
Students may not use laser pointers or pens
Use only lasers ≤ 5 mW
Do not allow direct or reflective eye exposure
Maintain bright ambient light to keep pupils constricted
Ionizing Radiation:
Only low-intensity sources (uranium, thorium, potassium, rubidium) are permitted
Keep sealed and avoid skin contact
Do not use powdered radioactive materials
A note on radiation sources: While the use of low-intensity radioactive sources remains permitted under current guidelines, teachers and schools are encouraged to limit their use and storage in favour of simulations or alternative activities whenever possible.
Static Electricity and Specialized Apparatus
Van de Graaff Generators:
Keep face and eyes away from discharge sphere
Students should wear glasses to reduce eye risk
Solvent and Spark Hazards:
No flammable solvents (e.g., ether, alcohol) near spark sources like spectral tubes or static discharge setups
Always check surroundings before activating spark-related devices