Principles of DNA Profiling (Antibiotic Resistance)
Pre-session preparation
There is no specific preparatory material for this session.
Learning outcomes
By the end of this session you should:
Understand the principles of antibiotic resitance
Gain hands-on experience of plating bacteria
Be able to perform simple dilution claculations
Gain hands on experience of using a spectrophotmeter
Be able to interpret results from a bacteria growth experiment
Activities
You will be working in groups of three and you will follow the instructions in the booklet below to complete a series of experiments
Resources & notes for laboratory staff
For the class:
one box each of different sized gloves
Bacteria grown overnight (Dom to make this)
6x spectrophotometers
In a blue tray (we will need 6 blue trays):
30 ul of Ampicillin 100 ug/ul in a labelled eppendorf
30 ul of Ampicillin 50 ug/ul in a labelled eppendorf
20 ul of Kanamycin 50 ug/ul in a labelled eppendorf
20 ul of Kanamycin 25 ug/ul in a labelled eppendorf
20 ul of Coffee in a labelled eppendorf
20 ul of Olive oil in a labelled eppendorf
20 ul of Hand sanitiser in a labelled eppendorf
20 ul of Tea in a labelled eppendorf
20 ul of Antibacterial spray in a labelled eppendorf
20 ul of Water in a labelled eppendorf
20 ul of Coke in a labelled eppendorf
20 ul of Diet coke in a labelled eppendorf
2x empty eppendorfs
All of the above should be in an Eppendorf rack
3x tweezers
Timer
2x P20 pipettes and tips
2x P1000 pippettes and tips
Tip waste container
3x LB agar plates
Ruler
Permanent fine tip Marker pen
6ml of labelled “overnight bacterial culture” in small glass bottles Dom to provide
10 ml of labelled water in small glass bottles
3x plastic plate spreaders
Timer
Glass bottle containing 15x autoclaved discs of filter paper
