Bacterial Transformation

Pre-session preparation

There is no specific preparatory material for this session.

Learning outcomes

By the end of this session you should be able to:

Discuss how bacteria attain antibiotic resistance

Use microbiological aseptc techniques, including the use of agar plates and broth

Transform E. coli with a plasmid that encoded a green fluorescent protein and antibiotic resistance

Explain the process of gene regulation

Activities

Your tutor will initially go through a PowerPoint presentation to introduce lthe sessions and safety protocols.

Following this, you will follow an instruction manual to dissect a sheep brain.

Below is a sample instruction manual and Powerpoint file

Bacterial Transformation presentation.pptx
student protocol.docx

Resources

Pipettes

Petri dishes

Marker pens

Eppendorf tubes

E. coli

Water bath


Plastic loops and spreaders

Transformation buffer

LB media

LB agar

pGLO plasmid

incubator


Ice buckets

Blue roll

Gloves

Lab coats

70 % ethanol

UV Torch

What our past attendees have done