Bacterial Transformation
Pre-session preparation
There is no specific preparatory material for this session.
Learning outcomes
By the end of this session you should be able to:
Discuss how bacteria attain antibiotic resistance
Use microbiological aseptc techniques, including the use of agar plates and broth
Transform E. coli with a plasmid that encoded a green fluorescent protein and antibiotic resistance
Explain the process of gene regulation
Activities
Your tutor will initially go through a PowerPoint presentation to introduce lthe sessions and safety protocols.
Following this, you will follow an instruction manual to dissect a sheep brain.
Below is a sample instruction manual and Powerpoint file
Resources
Pipettes
Petri dishes
Marker pens
Eppendorf tubes
E. coli
Water bath
Plastic loops and spreaders
Transformation buffer
LB media
LB agar
pGLO plasmid
incubator
Ice buckets
Blue roll
Gloves
Lab coats
70 % ethanol
UV Torch