Embedded Files
Summative Assessments for
Summative Assessments for
Unit Learning Targets (SEPs)
Unit Learning Targets (SEPs)
Analyze and interpret data from the pGLO lab to evaluate bacterial transformation. (SEP 5)
Construct an explanation regarding regulation of a eukaryotic or prokaryotic gene. (SEP 6)
Obtain, Evaluate, Communicate information regarding bacterial transformation(SEP8)
KUD ("Know, "Understand", Do")
KUD ("Know, "Understand", Do")
Unit 9 Gene Regulation KUD Sheet
General Unit & Activity Resources
General Unit & Activity Resources
Unit Slide Shows
Unit Slide Shows
OPERON GOOGLE SLIDE SHOW ("Know" II and III)
PACKAGING & EPIGENETICS GOOGLE SLIDE SHOW ("Know" IV and V)
RNAi GOOGLE SLIDE SHOW ("Know" VI)
Click here for operon graphic organizer and practice quiz
CONTENT-BASED TARGETS
CONTENT-BASED TARGETS
"Know" I- Explain the process of plasmid-based transformation of prokaryotic cells with a eukaryotic gene. Use the following terms: plasmid, rDNA (recombinant plasmid), eukaryotic gene of interest (ex: insulin or GFP), bacterium, eukaryotic cell, ampicillin resistance (ampR)gene, ampicillin, bacterial clones, pure culture, agar plate, mRNA, transformation
"Know" I- Explain the process of plasmid-based transformation of prokaryotic cells with a eukaryotic gene. Use the following terms: plasmid, rDNA (recombinant plasmid), eukaryotic gene of interest (ex: insulin or GFP), bacterium, eukaryotic cell, ampicillin resistance (ampR)gene, ampicillin, bacterial clones, pure culture, agar plate, mRNA, transformation
In vivo (using bacteria) gene cloning Animation
Target I: restriction enzymes, DNA cloning Quia quiz
"Know" II- Identify components of an operon model used to describe regulation of gene expression in prokaryotes. Use the following terms: promoter, regulatory gene mRNA, protein, RNA polymerase, operator, genes of operon
"Know" II- Identify components of an operon model used to describe regulation of gene expression in prokaryotes. Use the following terms: promoter, regulatory gene mRNA, protein, RNA polymerase, operator, genes of operon
Gene Expression and the order of the Operon by The Amoeba Sisters
"Know" III- Distinguish between inducible and repressible operon function in negative gene regulation. Provide examples of each type of operon. Use terms: co-repressor, active repressor, inactive repressor, no mRNA produced, mRNA produced, tryptophan, allolactose, lac operon, trp operon, inducer, “turn on”, “turn off”
"Know" III- Distinguish between inducible and repressible operon function in negative gene regulation. Provide examples of each type of operon. Use terms: co-repressor, active repressor, inactive repressor, no mRNA produced, mRNA produced, tryptophan, allolactose, lac operon, trp operon, inducer, “turn on”, “turn off”
(lac operon) McGraw Hill Quiz with animation
(trp operon) McGraw Hill Quiz with animation
Gene regulation Bozeman Science
"Know" IV- Explain how histone acetylation affects gene regulation in eukaryotic cells (use terms: histones, acetylate, stimulate transcription) and explain how DNA methylation affects gene regulation in eukaryotic cells (use terms: histones, methyl groups, repress transcription).
"Know" IV- Explain how histone acetylation affects gene regulation in eukaryotic cells (use terms: histones, acetylate, stimulate transcription) and explain how DNA methylation affects gene regulation in eukaryotic cells (use terms: histones, methyl groups, repress transcription).
Chromosomes, Histones, and Modification Video
Lick Your Rats--great explanation of Epigenetics and methylation
"Know" V- Describe how the organization of a typical eukaryotic gene lends itself to regulation. Use terms: enhancer, proximal control elements, distal control elements, promoter, exon, intron, poly-A signal sequence, termination region, activators, transcription factors, mediator proteins, RNA polymerase II, transcription initiation complex
"Know" V- Describe how the organization of a typical eukaryotic gene lends itself to regulation. Use terms: enhancer, proximal control elements, distal control elements, promoter, exon, intron, poly-A signal sequence, termination region, activators, transcription factors, mediator proteins, RNA polymerase II, transcription initiation complex
(Eukaryotic gene regulation and some review on prokaryotic) McGraw Hill multiple choice quiz, no animation
Eukaryotic Gene Regulation (McGraw Hill Animation)
Enhancers in Eukaryotic Gene Regulation
"Know" VI- Explain how the process of RNA interference (RNAi) is used to silence expression for certain genes. Use terms: miRNAs, Dicer, degrade mRNA, block translation, “turn off” gene expression
"Know" VI- Explain how the process of RNA interference (RNAi) is used to silence expression for certain genes. Use terms: miRNAs, Dicer, degrade mRNA, block translation, “turn off” gene expression
RNAi Video from the journal Nature
TedEd RNAi: Slicing, dicing, and serving your cells
RNAi Notes
Nova ScienceNow RNAi Video From Class
RNAi Viewing Guide (NovaScienceNow) Worksheet & Answers
PROCESS-BASED TARGETS
PROCESS-BASED TARGETS
"Do" A- Transform E. coli bacteria with a recombinant DNA plasmid containing the GFP gene to alter the phenotype of bacteria cells under UV light.
Report abuse