Part 3: Extraction With An Aqueous Base

Testing the pH of the Aqueous Layer

Combine the aqueous layers in a single flask labeled G. Test the pH of solution G. Designate the organic layer as A3 and spot a sample in the fourth lane of the TLC plate.

This video shows a small flask with a colorless liquid inside. A gloved hand uses a tweezers to dip a piece of pH paper into the liquid and holds the pH paper up to the camera. The pH paper has turned dark green in color.

A photo of a piece of pH paper along with the color key. The dark green color corresponds to a pH of 9.

A photo of a TLC plate illuminated by UV light with four spots visible along the baseline labeled A1, C, A2, and A3.

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Workup the organic layer

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