Create a series of solutions of varying concentrations (5, 10, 15, 20 and 40 µM) using a stock solution of curcumin with a known concentration. Measure the absorbance of these solutions and plot the resulting data to create a calibration curve.
Extract curcumin from turmeric using ethanol. Measure the absorbance of your ethanol sample and determine the concentration of curcumin by comparing the absorbance to the calibration curve.
Design an experiment that investigates how one variable affects the extraction efficiency of curcumin from turmeric.