Lab Publications

Transcription start site heterogeneity and its role in RNA fate determination distinguish HIV-1 from other retroviruses and are mediated by core promoter elements. Kharytonchyk S, Burnett C, Gc K, Telesnitsky A. (2023) bioRxiv May 22:2023.05.22.541776. doi: 10.1101/2023.05.22.541776. Preprint. PMID: 37292892

Bimodal Expression Patterns, and Not Viral Burst Sizes, Predict the Effects of Vpr on HIV-1 Proviral Populations in Jurkat Cells. Edmond Atindaana, Abena Kissi-Twum , Sarah Emery, Cleo Burnett, Jake Pitcher, Myra Visser, Jeffrey M Kidd, Alice Telesnitsky (2022) mbio Apr 26;13(2):e0374821. doi: 10.1128/mbio.03748-21. Epub 2022 Apr 6.

5'-Cap sequestration is an essential determinant of HIV-1 genome packaging.

Ding P, Kharytonchyk S, Kuo N, Cannistraci E, Flores H, Chaudhary R, Sarkar M, Dong X, Telesnitsky A, Summers MF. (2021)

Proc Natl Acad Sci U S A. 2021 Sep 14;118(37):e2112475118. doi: 10.1073/pnas.2112475118.

PMID: 34493679 

Stability and conformation of the dimeric HIV-1 genomic RNA 5'UTR.

Blakemore RJ, Burnett C, Swanson C, Kharytonchyk S, Telesnitsky A, Munro JB. (2021)

Biophys J. 2021 Nov 2;120(21):4874-4890. doi: 10.1016/j.bpj.2021.09.017. Epub 2021 Sep 14.

PMID: 34529947

Identification of the initial nucleocapsid recognition element in the HIV-1 RNA packaging signal.

Ding P, Kharytonchyk S, Waller A, Mbaekwe U, Basappa S, Kuo N, Frank HM, Quasney C, Kidane A, Swanson C, Van V, Sarkar M, Cannistraci E, Chaudhary R, Flores H, Telesnitsky A, Summers MF. (2020)

Proc Natl Acad Sci U S A. 2020 Jul 28;117(30):17737-17746. doi: 10.1073/pnas.2008519117. 

HIV-1 spliced RNAs display transcription start site bias. Esquiaqui JM, Kharytonchyk S, Drucker D, Telesnitsky A. (2020) RNA. 26(6):708-714. doi: 10.1261/rna.073650.119. 

Structural basis for transcriptional start site control of HIV-1 RNA fate. Brown JD, Kharytonchyk S, Chaudry I, Iyer AS, Carter H, Becker G, Desai Y, Glang L, Choi SH, Singh K, Lopresti MW, Orellana M, Rodriguez T, Oboh U, Hijji J, Ghinger FG, Stewart K, Francis D, Edwards B, Chen P, Case DA, Telesnitsky A, Summers MF. (2020) Science. 368(6489):413-417. doi: 10.1126/science.aaz7959. 

Stable integrant-specific differences in bimodal HIV-1 expression patterns revealed by high-throughput analysis. Read DF, Atindaana E, Pyaram K, Yang F, Emery S, Cheong A, Nakama KR, Burnett C, Larragoite ET, Battivelli E, Verdin E, Planelles V, Chang CH, Telesnitsky A, Kidd JM. (2019) PLoS Pathog 15(10):e1007903.

Flexibility in nucleic acid binding is central to APOBEC3H antiviral activity. Bohn JA, DaSilva J, Kharytonchyk S, Mercedes M, Vosters J, Telesnitsky A, Hatziioannou T, Smith JL. (2019) J Virol. pii: JVI.01275-19.

Multiple, Switchable Protein:RNA Interactions Regulate Human Immunodeficiency Virus Type 1 Assembly.  Bieniasz P, Telesnitsky A.  (2018) Annu Rev Virol. 10.1146

Influence of gag and RRE Sequences on HIV-1 RNA Packaging Signal Structure and Function. Kharytonchyk S, Brown JD, Stilger K, Yasin S, Iyer AS, Collins J, Summers MF, Telesnitsky A. (2018) J Mol Biol. 430(14):2066-2079

Not just Salk. Greider C, Hopkins N, Steitz J, Amon A, Asai D, Barres B, Bass B, Bassler B, Birgeneau R, Bjorkman P, Botchan M, Brugge J, Cech T, Colwell R, Craig N, deLange T, Eisen M, Gottesman S, Green R, Handelsman J, Kimble J, King MC, Lehmann R, Marder E, Mullins D, O'Shea E, Schmid S, Seydoux G, Spradling A, Storz G, Szostak J, Telesnitsky A, Tilghman S, Tjian R, Vale R, Wolberger C, Zakian V. (2017) Science. 357(6356):1105-1106.

Transcriptional start site heterogeneity modulates the structure and function of the HIV-1 genome. Kharytonchyk, S., Monti, S., Smaldino, P., Van, V., Bolden, N.C., Russo, E., Shuey, A., Swanson, C., Brown, J., Telesnitsky, A. and Summers, M.F. (2016) Proc Natl Acad Sci U S A. 113:13378-13383.

Analysis of the human immunodeficiency virus-1 RNA packageome.  Eckwahl, M.J., Arnion, H., Kharytonchyk, S., Zang, T., Bieniasz, P.D., Telesnitsky, A. and Wolin, S.L. (2016) RNA 22:1228-38.

The Host RNAs in Retroviral Particles. Telesnitsky A and Wolin SL. (2016) Viruses 8: E235.

Resolution of Specific Nucleotide Mismatches by Wild-Type and AZT-Resistant Reverse Transcriptases during HIV-1 Replication. Kharytonchyk, S., King, S.R., Ndongmo, C.B., Stilger, K.L., An, W. and Telesnitsky, A. (2016) J Mol Biol 428:2275-88.

Host RNA Packaging by Retroviruses: A Newly Synthesized Story  Eckwahl MJ, Telesnitsky A, and Wolin SL. (2016) MBio 7:e02025-15.

Structure of the HIV-1 RNA packaging signal  Keane SC, Heng X, Lu K, Kharytonchyk S, Ventateswaran R, Carter G, Barton S, Hosic A, Florwick A, Santos J, Bolden NC, McCowin S, Case DA, Johnson B, Salemi M, Telesnitsky A, Summers MF. (2015) Science 348:971-921.

A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway. Eckwahl MJ, Sim S, Smith D, Telesnitsky A, Wolin SL. (2015) Genes and Development 29:646-657.

Determinants of Moloney murine leukemia virus Gag-Pol and genomic RNA proportions. (J. Virol.-2014-Johnson-7267-75.pdf, 04-16-2014) Johnson SF, Collins JT, D'Souza VM, Telesnitsky A. (2014) J Virol. 88(13):7267-75.  

Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.  Johnson SF1, Garcia EL, Summers MF, Telesnitsky A. (2012)  J Virol. 430(2):100-9.

cis-Acting determinants of 7SL RNA packaging by HIV-1. Keene SE, Telesnitsky A. (2012) J Virol. 86(15):7934-42.

Identification of a minimal region of the HIV-1 5'-leader required for RNA dimerization, NC binding, and packaging.  Heng X1, Kharytonchyk S, Garcia EL, Lu K, Divakaruni SS, LaCotti C, Edme K, Telesnitsky A, Summers MF.  (2012) J Mol Biol. 417(3):224-39.

NMR detection of structures in the HIV-1 5'-leader RNA that regulate genome packaging.  Lu K1, Heng X, Garyu L, Monti S, Garcia EL, Kharytonchyk S, Dorjsuren B, Kulandaivel G, Jones S, Hiremath A, Divakaruni SS, LaCotti C, Barton S, Tummillo D,Hosic A, Edme K, Albrecht S, Telesnitsky A, Summers MF. (2011) Science.1210460.

7SL RNA Is Retained in HIV-1 Minimal Virus-Like Particles as an S-Domain Fragment.  Keene, S.E., King, S.R. and Telesnitsky, A. (2010) J. Virol.84:9070-77.

Retroviral RNA dimerization and packaging: the what, how, when, where, and why.  Johnson, S. F. and Telesnitsky, A. (2010) . PLoS Pathogens 6(10): e1001007.  

An RNA structural switch regulates diploid genome packaging by Moloney murine leukemia virus.   Miyazaki, Y., Garcia, E.L., King, S.R., Iyalla, K. Loeliger, K., Starck, P., Syed, S., Telesnitsky, A., and Summers, M.F. (2010) J. Mol. Biol. 396:141-152

The remarkable frequency of HIV-1 genetic recombination.   Onafuwa-Nuga, A. and Telesnitsky, A. (2009) MMBR 73: 451-479.  

7SL RNA, but not the 54kd signal recognition protein, is an abundant component of both infectious HIV-1 and defective virus-like particles  Onfuwa-Nuga, A.A., Telesnitsky, A. and King. S.R. (2006) RNA 12:542-546.

Two distinct Moloney murine leukemia virus RNAs produced from a single locus dimerize at random.  Flynn and Telesnitsky, (2006) Virology. 344: 391-400.  

Nonrandom packaging of host RNAs in Moloney murine leukemia virus. Onafuwa-Nuga, King, and Telesnitsky, A. (2005) J Virol.  79(21):13528-37

Non-random dimerization of murine leukemia virus genomic RNAs.   Flynn, J.A., An, W., King, S.R., Telesnitsky, A. (2004) J. Virol. 78:12129-12139. 

Transductive recombination can occur frequently and in proportion to polyadenylation signal readthrough.  An, W. and Telesntisky, A. (2004) J. Virol 78:. 3419-3428. 

HIV-1 genetic recombination is more frequent than that of Moloney murine leukemia virus despite similar template switching rates  Onafuwa, A., An, W., Robson, N., and Telesnitsky, A. (2003) J. Virol 77(8): 4577-458. 

Mismatch extension during strong stop strand transfer and minimal homology requirements for replicative template switching during Moloney murine leukemeia virus replication.  Marr, S.F. and Telesnitsky, A. (2003) J. Mol. Biol. 330:657-674. 

Ribonuclease H activity is required for high frequency repeat deletion during Moloney murine leukemia virus replication.  Brincat, J., Pfeiffer, J.K., and Telesnitsky, A. (2002) J.Virol 76:88-95. 

Effects of varying sequence similarity on the frequency of repeat deletion during reverse transcription of a human immunodeficiency virus type 1 vector.  An, W. and Telesnitsky, A. (2002) J. Virol. 76:7897-902. 

HIV-1 genetic recombination: experimental approaches and observations.   An, W. and Telesnitsky, A. (2002) AIDS Reviews 4:195-212. 

Effects of limiting homology at the site of intermolecular recombinogenic switching during Moloney murine leukemia virus replication. Pfeiffer, J.K. and Telesnitsky, A. (2001) J. Virol 75:11263-11274. 

Frequency of direct repeat deletion in a human immunodeficiency virus type 1 vector during reverse transcription in human cells.  An, W. and Telesnitsky, A. (2001) Virology 286:475-482. 

Selection of optimal polypurine tract region sequences during Moloney murine leukemia virus replication.  Robson, N.D. and Telesnitsky, A. (2000) J Virol 74:10293-10303.

Replication of lengthened Moloney murine leukemia virus genomes is impaired at multiple stages.  Shin, N-H, Hartigan-O'Connor, D., Pfeiffer, J.K., and Telesnitsky, A. (2000)J. Virol 74: 2694-2702. 

Structure-based Moloney murine leukemia virus reverse transcriptase mutants with altered intracellular direct repeat deletion frequencies.  Pfeiffer, J.K., Georgiadis, M.M., and Telesnitsky, A. (2000) J. Virol 74(20): 9629- 36. 

The effects of 3' untranslated region mutations on plus strand priming during Moloney murine leukemia virus replication.  Robson, N.D. and Telesnitsky, A. (1999) J. Virol 73: 948-957. 

Altering the intracellular environment increases the frequency of tandem repeat deletion during Moloney murine leukemia virus reverse transcription.  Pfeiffer, J.K.,Topping, R.S., Shin, N-H, and Telesnitsky, A. (1999) J. Virol 73 (10): 8441-8447. 

Cis-acting elements required for strong stop acceptor template selection during Moloney murine leukemia virus reverse transcription. Topping, R. Demoitie, M-A. Shin, N.H. and Telesnitsky, A. (1998) J. Mol Biol. 281: 1-15. 

Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors.  Kulpa, D. Topping, R. and Telesnitsky, A. (1997) EMBO J 16: 856-865. 

Reverse transcriptase and the generation of retroviral DNA.  Telesnitsky, A and Goff, S.P. (1997) In Retroviruses, Coffin, J., Varmus, H., and Hughes, S., eds. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. pp.121-160.