References for generating, editing or differentiating human pluripotent stem cells (hPSCs):
1. Okita, K., et al., A more efficient method to generate integration-free human iPScells. Nature Methods, 2011. 8(5): p. 409-12
2. Ran, F.A., et al., Genome engineering using the CRISPR-Cas9 system. Nature Protocols, 2013. 8: p2281–2308
3. Santos, D.P., et al., Comprehensive protocols for CRISPR/Cas9-based gene editing inhuman pluripotent stem cells. Curr. Protoc. Stem Cell Biol, 2016. 38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15
4. Shi, Y., et al., Human Cerebral Cortex Development from Pluripotent Stem Cells toFunctional Excitatory Synapses. Nature Neurosci., 2012. 15(3): p.477-486
5. Mariani, J., et al., FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders. Cell, 2015. 162(2): p.375-90
6. Qian, X., et al., Brain -Region-specific Organoids Using Mini-bioreactors for Modeling Zikv Exposure. Cell, 2016, 165, 1-17
Online CRISPR/Cas9 design tools:
1. the CRISPR Design Tool (crispr.mit.edu) (Hsu et al., 2013)
2. ZiFiT targeter (zifit.partners.org/ZiFiT) (Fu et al., 2014)
3. CasFinder (arep.med.harvard.edu/CasFinder/) (Aach, Mali, & Church, 2014)
4. E-Crisp (www.e-crisp.org/E-CRISP/) (Heigwer, Kerr, & Boutros, 2014)
5. Benchling: https://benchling.com/crispr
Protocols from the HSCGE core:
1. iPSC reprogramming:
2. iPSC maintenance:
3. 2D & 3D Neural differentiation: