Useful Protocols

Some useful protocols

Simple generalized immunostaining protocol (immunostaining.pdf, 27.0 kb, 06-06-2006)

Ringer's buffer recipe (ringer_s_buffer.pdf, 12.0 kb, 06-01-2007)

We recommend this buffer for live-cell imaging. It is buffered with HEPES against pH changes, contains glucose to keep the cells happy, and does not contain any phenol red or other fluorescent compounds to interfere with quantitative imaging. Combined with temperature control, primary macrophages can be kept happy for over an hour in this buffer.

This works for almost any primary-secondary antibody combination. Perfected in the Margolis lab at UM when Sam was a post-doc.