Deconvolution: 3D image reconstruction

Three-dimensional image data collected from the CLCI microscopes (the 4D FRET microscope and the confocal laser-scannning microscope) frequently requires reconstruction to restore fine details to the image that are lost by optical blurring. This distortion of the image is a measureable, inherent property of the optics of the microscope.

The image reconstruction process is called deconvolution and it applies mathematical formulae related to properties of the microscope optical path (including the objective, for example) to reassign the blurred light in the collected image in order to provide a more accurate estimate of the original object.

The CLCI maintains a license for the Huygens software package, created by Scientific Volume Imaging b.v., for three-dimensional image deconvolution. This software is run on our applications server, accessible through the workstations in the CLCI and remotely by permission.

The CLCI provides hands-on training in three-dimensional image acquisition and analysis, including deconvolution. As a supplement to this training, SVI provides excellent online documentation regarding their software package:

Tutorials and general information regarding acquisitions and deconvolution can be found here

Online user documentation (PDFs) can be found here. If you are intersted in using this software, we suggest you read with the Huygens Essential followed by the Huygens Professional guides. The Scripting and Administration packages are unnecessary or inaccessible to the vast majority of our users.