Presentations
The following presentations are meant to provide you with a background in a number of subjects related to Structural Biology including protein expression, structure refinement and structure validation.
You do not need to fear protein expression. This presentation aims to make protein expression easier by describing easy modifications to your media that will enable you to achieve higher cell densities for protein expression in E. coli. Higher cell densities = more protein. More protein = SUCCESS.
This presentation details the application of side chain torsion angle potential of mean force and hydrogen bond database potential of mean force to protein structure refinement in XPLOR-NIH. The inner workings of each potential are discussed, a ready-to-run script is presented. Additionally, recent application leading to substantial improvements to the Lim5 NMR structure are shown as an example of the capabilities of the improved refinement script.
This presentation covers the basics of structure validation for both NMR and X-ray crystallography. Resources for structure validation are presented followed by a short MolProbity tutorial.
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