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Genomics: Information flow in biological systems
Mitali Mukerji (mitali@igib.res.in), Siva Ramalingam, Debojyoti Chakraborty
The course deals with an overview of the science of genomics and the changing paradigms in genetics and biology; introduction to human genome project and the knowledge that transpired from it, Next Generation Sequencing Technologies and its applications, insights from sequencing of diverse organisms; multi-omic technologies and its role in understanding the structure- function organization of the human genome; genomics in diverse areas of science from basic biology to application, variability in the human genome, methodologies and its role in health and disease, modelling and correction using genome editing tools, Functional annotation of the human genome, overview of the Encode /Epigenome projects and its applications in stem cell therapeutics
Item wise details to be covered as a part of the course:
Introduction to genomics and its applications
The Human Genome Project: Genesis, Strategy, Tools and Insights
Next Generation Sequencing Technology: Development and Applications
Insights from diverse genome sequencing projects
Changing paradigms in Biology: Abundance and Role of noncoding RNAs, repetitive sequences and pseudogenes in the human genome
Functional Annotation of the Human genome: overview of the Encode and Epigenome project
The Human Microbiome project
Variability in the human genome: The HapMap and 1000 genomes and Indian Genome Variation project
Functional genomics for dissection of genotype to phenotype studies
Overview of mammalian gene editing from Site Specific Nucleases to the recently developed CRISPR Cas9 system
Salient features of the individual genome editing tools
Gene editing for therapeutic applications
Role of gene editing in generation of cellular models of disease and in regenerative medicine
The pitfalls of genome editing and its safety issues
Optimization of CRISPR and development of alternate editing tools
iPSC culture, its implications and current limitations
Stem cell therapeutics and the way ahead
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