業績

論文

1. SALL1 functions as a tumor suppressor in hepatocellular carcinoma cells.

Saito Y, Miyakawa S, Ando S, Kasano-Camones CI, Sumardika IW, Obayashi A, Morimoto A, Kurosawa A, Sakaguchi M, Ninomiya K, Inoue Y.

Biochem Biophys Res Commun. 778:152385, 2025.

2. Sulforaphane induces transient reactive oxygen species-mediated DNA damage in HeLa cells.

Kobayashi S, Nishiba S, Sato C, Toriumi K, Someya Y, Adachi N, Takeda S, Kurosawa A.

Genes Cells. 2025 Jan;30(1):e13190. doi: 10.1111/gtc.13190.

3. Quercetin exhibits cytotoxicity in cancer cells by inducing two-ended DNA double-strand breaks.

Someya Y, Saito S, Takeda S, Adachi N, Kurosawa A.

Biochem Biophys Res Commun. 2024 Dec 20;739:150977. doi: 10.1016/j.bbrc.2024.150977. Epub 2024 Nov 13.

4. Arsenic affects homologous recombination and single-strand annealing but not end-joining pathways during DNA double-strand break repair.

Kurosawa A, Saito S, Sakurai M, Shinozuka M, Someya Y, Adachi N.

FEBS J. 2023 Nov;290(22):5313-5321. doi: 10.1111/febs.16922.

5. A cell system-assisted strategy for evaluating the natural anti-oxidant-induced double-stranded DNA break (DSB) style.

Someya Y, Kobayashi S, Toriumi K, Takeda S, Adachi N, Kurosawa A.

Genes. 2023 Feb;14(2):420.

6. Complex genetic interactions between DNA polymerase β and the NHEJ ligase.

  Kurosawa A,  Kuboshima H, Adachi N.

FEBS J. 2020 Jan;287(2):377-385. doi: 10.1111/febs.15012. Epub 2019 Jul 29.

7. GPR31 and GPR151 are activated under acidic conditions.

Mashiko M, Kurosawa A, Tani Y, Tsuji T,  Takeda S.

J Biochem. 2019 Oct 1;166(4):317-322. doi: 10.1093/jb/mvz042.

8. A novel partial agonist of GPBA reduces blood glucose level in a murine glucose tolerance test.

Enomoto R, Kurosawa A, Nikaido Y, Mashiko M, Saheki T, Nakajima N, Kuroiwa S, Otobe M, Ohsaki M, Tooyama K, Inoue Y, Kuwabara N, Kikuchi O, Kitamura T, Kojima I, Nakagawa Y, Saito T, Osada H, Futahashi M, Sezutsu H, Takeda S.

Eur J Pharmacol. 2017 Nov 5;814:130-137. doi: 10.1016/j.ejphar.2017.08.017. Epub 2017 Aug 17.

9. Mechanistic basis for increased human gene targeting by promoterless vectors-roles of homology arms and Rad54 paralogs.

Saito S, Kurosawa A, Adachi N.

FEBS J. 2017 Sep;284(17):2748-2763. doi: 10.1111/febs.14137. Epub 2017 Jul 6.

10. Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain.

Yanai T, Kurosawa A, Nikaido Y, Nakajima N, Saito T, Osada H, Konno A, Hirai H, Takeda S.

Genes Cells. 2016 Jul;21(7):717-27. doi: 10.1111/gtc.12378. Epub 2016 May 17.

11. In vivo and in vitro evaluation of novel μ-opioid receptor agonist compounds.

Nikaido Y, Kurosawa A, Saikawa H, Kuroiwa S, Suzuki C, Kuwabara N, Hoshino H, Obata H, Saito S, Saito T, Osada H, Kobayashi I, Sezutsu H, Takeda S.

Eur J Pharmacol. 2015 Nov 15;767:193-200. doi: 10.1016/j.ejphar.2015.10.025. Epub 2015 Oct 20.

12. Role for Artemis nuclease in the repair of radiation-induced DNA double strand breaks by alternative end joining.

Moscariello M, Wieloch R, Kurosawa A, Li F, Adachi N, Mladenov E, Iliakis G.

DNA Repair (Amst). 2015 Jul;31:29-40. doi: 10.1016/j.dnarep.2015.04.004. Epub 2015 Apr 25.

13. Analysis of the role of homology arms in gene-targeting vectors in human cells.

Ishii A, Kurosawa A, Saito S, Adachi N.

PLoS One. 2014 Sep 24;9(9):e108236. doi: 10.1371/journal.pone.0108236. eCollection 2014.

14. DNA ligase IV and Artemis act cooperatively to suppress homologous recombination in human cells: implications for DNA double-strand break repair.

Kurosawa A, Saito S, So S, Hashimoto M, Iwabuchi K, Watabe H, Adachi N.

PLoS One. 2013 Aug 14;8(8):e72253. doi: 10.1371/journal.pone.0072253. eCollection 2013.

15. Endogenous factors causative of spontaneous DNA damage that leads to random integration in human cells.

Kamekawa H, Kurosawa A, Umehara M, Toyoda E, Adachi N.

Gene Technology. 2013 2:1000105.

16. Interference in DNA replication can cause mitotic chromosomal breakage unassociated with double-strand breaks.

Fujita M, Sasanuma H, Yamamoto KN, Harada H, Kurosawa A, Adachi N, Omura M, Hiraoka M, Takeda S, Hirota K.

PLoS One. 2013;8(4):e60043. doi: 10.1371/journal.pone.0060043. Epub 2013 Apr 3.

17. Both CpG methylation and activation-induced deaminase are required for the fragility of the human bcl-2 major breakpoint region: implications for the timing of the breaks in the t(14;18) translocation.

Cui X, Lu Z, Kurosawa A, Klemm L, Bagshaw AT, Tsai AG, Gemmell N, Müschen M, Adachi N, Hsieh CL, Lieber MR.

 Mol Cell Biol. 2013 Mar;33(5):947-57. doi: 10.1128/MCB.01436-12. Epub 2012 Dec 21.

18. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs.

Malu S, De Ioannes P, Kozlov M, Greene M, Francis D, Hanna M, Pena J, Escalante CR, Kurosawa A, Erdjument-Bromage H, Tempst P, Adachi N, Vezzoni P, Villa A, Aggarwal AK, Cortes P.

J Exp Med. 2012 May 7;209(5):955-63. doi: 10.1084/jem.20111437. Epub 2012 Apr 23.

19. Nucleofection-based gene targeting in human pre-B cells.

Kurosawa A, Saito S, Mori M, Adachi N.

Gene. 2012 Jan 15;492(1):305-8. doi: 10.1016/j.gene.2011.11.003. Epub 2011 Nov 18.

20. Heterozygous disruption of the DNA topoisomerase I gene confers cellular resistance to camptothecin in human cells.

Toyoda E, Kurosawa A, Fujii M, Adachi N.

Biol Pharm Bull. 2009 Apr;32(4):724-7.

21. Topoisomerase IIalpha inhibition following DNA transfection greatly enhances random integration in a human pre-B lymphocyte cell line.

Toyoda E, Kurosawa A, Kamekawa H, Adachi N.

Biochem Biophys Res Commun. 2009 May 8;382(3):492-6. doi: 10.1016/j.bbrc.2009.03.047. Epub 2009 Mar 13.

22. Impact of non-homologous end-joining deficiency on random and targeted DNA integration: implications for gene targeting.

Iiizumi S, Kurosawa A, So S, Ishii Y, Chikaraishi Y, Ishii A, Koyama H, Adachi N.

Nucleic Acids Res. 2008 Nov;36(19):6333-42. doi: 10.1093/nar/gkn649. Epub 2008 Oct 3.

23. NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform.

Toyoda E, Kagaya S, Cowell IG, Kurosawa A, Kamoshita K, Nishikawa K, Iiizumi S, Koyama H, Austin CA, Adachi N.

 J Biol Chem. 2008 Aug 29;283(35):23711-20. doi: 10.1074/jbc.M803936200. Epub 2008 Jul 2.

24. Cell sorting analysis of cell cycle-dependent X-ray sensitivity in end joining-deficient human cells.

Iwabuchi K, Hashimoto M, Matsui T, Kurosawa A, Adachi N, Date T.

Biochem Biophys Res Commun. 2008 Aug 8;372(4):662-7. doi: 10.1016/j.bbrc.2008.05.147. Epub 2008 Jun 4.

25. Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6.

Adachi N, Kurosawa A, Koyama H.

Methods Mol Biol. 2008;435:17-29. doi: 10.1007/978-1-59745-232-8_2.

26. The requirement of Artemis in double-strand break repair depends on the type of DNA damage.

Kurosawa A, Koyama H, Takayama S, Miki K, Ayusawa D, Fujii M, Iiizumi S, Adachi N.

DNA Cell Biol. 2008 Jan;27(1):55-61.

27. Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae.

Takayama S, Fujii M, Kurosawa A, Adachi N, Ayusawa D.

Curr Genet. 2007 Nov;52(5-6):203-11. Epub 2007 Sep 27.

28. Human neutrophils isolated from peripheral blood contain Ku protein but not DNA-dependent protein kinase.

Kurosawa A, Shinohara K, Watanabe F, Shimizu-Saito K, Koiwai O, Yamamoto K, Teraoka H.

Int J Biochem Cell Biol. 2003 Jan;35(1):86-94.

総説（英文）

1. Autophosphorylation and self-activation of DNA-dependent protein kinase.

Kurosawa A.

Genes (Basel). 2021 Jul 19;12(7):1091. doi: 10.3390/genes12071091. Review.

2. Mutations in  XRCC4 cause primordial dwarfism without causing immunodeficiency.

Saito S, Kurosawa A, Adachi N.

 J Hum Genet. 2016 May 12. doi: 10.1038/jhg.2016.46. [Epub ahead of print] Review.

3. Functions and regulation of Artemis: a goddess in the maintenance of genome integrity.

 Kurosawa A, Adachi N.

J Radiat Res. 2010;51(5):503-9. Epub 2010 Jun 11. Review.

総説・解説（和文）

1. 非相同末端連結とその阻害剤

黒沢 綾, 足立 典隆.

放射線生物研究. 52:162-272, 2017. （総説）

2. XRCC4遺伝子変異を原因とする遺伝性疾患：免疫不全を伴わない原発性小人症.

斎藤 慎太, 黒沢 綾, 足立 典隆.

放射線生物研究. 50:269-280, 2015. （総説）

3. ヒト細胞におけるDNA二本鎖切断の修復.

黒沢 綾, 足立 典隆.

アイソトープニュース. 721:8-14, 2014. （解説）

4. ヒト染色体DNA切断の修復機構．

足立 典隆，矢野 憲一，黒沢 綾．

蛋白質核酸酵素．共立出版． 54:472-478, 2009. （総説）

5. ヒト重症複合型免疫不全症候群原因遺伝子ARTEMISの機能と制御．

黒沢 綾，足立 典隆．

放射線生物研究. 42:386-401, 2007. （総説）

6. DNA二重鎖切断修復の新守護神．

黒沢 綾，足立 典隆．

実験医学．羊土社． 24:1304-1305, 2006. （解説）

その他

1. ダルムシュタット独国工科大学Löbrich研究室での研修報告

黒沢 綾.

放射線生物研究. 56:408-410, 2021. （報告）

2. Repair of accidental DNA double-strand breaks in the huma genome and its relevance to vector DNA integration.

Adachi N, Saito S, Kurosawa A.

Gene Technology 3:e107, 2013. (Editorial)