BCA

BCA solution: 1ml Cu2So4 (4% in H2O) to 49 ml BCA

Analyse 2 µl lysate per sample in 18 µl H2O

Add 180 µl of BCA sol

Incubate 30 min a 60° C or 45 min at 37°C

Read at 562 nm.

Standard curve: 1,2,4,6,8,10,20 µg bsa.