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Liquid-liquid phase separation (LLPS) to engineer synthetic membraneless organelle in bacterial cells
Intrinsically disordered proteins can undergo liquid-liquid phase separation (LLPS) to form densely concentrated biomolcular condensates
These condensates can function as synthetic membrane-less organelles (MLOs) inside bacterial cells that can compartmentalize metabolic reactions
We are exploring tardigrade-derived disordered protein CAHS to engineer MLOs inside bacterial cells to achieve efficient bioproduction from microbial cell factory
Engineering functional protein nano-biomaterials
Engineering of ultrastable self-assembling protein gamma-prefoldin from extremophile M. jannaschii
Redesign protein-protein interaction interface for the controlled assembly into defined geometric shapes
Functionalization of nanofibers through attaching enzymes or metal nanoparticles to form biocatalytic / electronic nanofibers
Evolving protein materials
Protein functionality can be improved through artifical laboratory-assisted evolution (directed evolution)
While evolution of enzymes has been widely demonstrated, evolution of protein material assembly is largely unexplored
We aim to use novel platform such as phage-assisted continuous evolution (PACE) to evolve material properties and assembly behaviors of proteins
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