Engineering Noncanonical Cofactors To Expand Cellular Functions

S. Lim and S. Banta 

ACS Synthetic Biology, 2025. Link

Comparative evaluation of cell-based assay technologies for scoring drug-induced condensation of SARS-CoV-2 nucleocapsid protein

R. T. Quek, C. R. Shirazinejad, C. L. Young, K. S. Hardy, S. Lim, P. J. Elms, D. T. McSwiggen, T. J. Mitchison and P. A. Silver

SLAS Discovery 2025, 31, 100220. Link

Enabling high-throughput enzyme discovery and engineering with a lowcost, robot-assisted pipeline

B. Norton-Baker, M. C. R. Denton, N. P. Murphy, B. Fram, S. Lim, E. Erickson, N. P. Gauthier and G. T. Beckham

Scientific Reports 2024, 14 (1) 14449. Link

Tardigrade secretory proteins protect biological structures from desiccation

S. Lim, C. B. Reily, Z. Barghouti, B. Marelli, J. C. Way and P. A. Silver

Communications Biology 2024, 7 (1) 633. Link

Simultaneous enhancement of multiple functional properties using evolution-informed protein design

B. Fram, I. Truebridge, Y. Su, A. J. Riesselman, J. B. Ingraham, A. Passera, E. Napier, N. N. Thadani, S. Lim, K. Roberts, G. Kaur, M. Stiffler, D. S. Marks, C. D. Bahl, A. R. Kahn, C. Sander and N. P. Gauthier

Nature Communications 2024, 15 (1) 5141. Link

Phase-separated biomolecular condensates for biocatalysis

S. Lim and D. S. Clark

Trends in Biotechnology 2024, 42 (4) 496-509. Link

High spontaneous integration rates of end-modified linear DNAs upon mammalian cell transfection

S. Lim*, R. R. Yocum, P. A. Silver and J. C. Way

Scientific Reports 2023, 13 (1) 6835. (*Co-corresponding author) Link

Natural and designed proteins inspired by extremotolerant organisms can form condensates and attenuate apoptosis in human cells

M. T. Veling, D. T. Nguyen, N. N. Thadani, M. E. Oster, N. J. Rollins, K. P. Brock, N. P. Bethel, S. Lim, D. Baker,  J. C. Way, D. S. Marks, R. L. Chang and P. A. Silver

ACS Synthetic Biology 2022, 11 (3) 1292-1302. Link

Filamentous chaperone protein-based hydrogel stabilizes enzymes against thermal inactivation

D. Xu, S. Lim, Y. Cao, A. Abad, A. N. Kang and D. S. Clark

Chemical Communications 2021, 57 (45) 5511-5513. Link

CRISPR/Cas-directed programmable assembly of multi-enzyme complexes

S. Lim, J. Kim, Y. Kim, D. Xu and D. S. Clark

Chemical Communications 2020, 56 (36) 4950-4953. Link

Production of multicomponent protein templates for the positioning and stabilizzation of enzymes

S. Lim, D. S. Clark and D. J. Glover

Methods in Molecular Biology 2020, 2073, 101-115. Link

Enhanced enzyme activity through scaffolding on customizable self-assembling protein filaments

S. Lim, G. A. Jung, D. J. Glover and D. S. Clark

Small 2019, 15 (20), 101-115. Link 

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Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform

S. Lim, G. A. Jung, R. Muckom, D. J. Glover and D. S. Clark

Chemical Communications 2019, 55 (6) 806-809. Link

Assembly of multicomponent protein filaments using engineered subunit interfaces

D. J. Glover, S. Lim, D. Xu, N. B. Sloan. Y. Zhang and D. S. Clark

ACS Synthetic Biology 2018, 7 (10) 2447-2456. Link

Prefoldins in archaea

S. Lim, D. J. Glover and D. S. Clark

Advances in Experimental Medicine and Biology 2018, 1106, 11-23. Link