研究方向
Research Direction

更新日期 2026.07.23

中文

1. 本實驗室在台灣建立唯一以氣相層析/負離子化學游離質譜儀(gas chromatography/negative-ion chemical-ionization mass spectrometry; GC/NICI-MS)分析人和小鼠之體液及小鼠組織中F2-isoprostanes、isofurans、F4-neuroprostanes等分子之平台。這些分子為活體中之脂質過氧化之黃金標準指標，且我們將此平台用於研究氧化傷害在各式人類疾病或毒性的角色。目前已和林口長庚醫師合作而應用到此平台的研究主題有蜘蛛膜下腔出血（常見之出血性中風）、頭部外傷、老人癡呆症、除草劑巴拉刈(paraquat)中毒之免疫抑制治療、和鉛(Pb)及汞(Hg)中毒之螯合治療。

2. 我們在人類細胞株或含粒線體DNA (mtDNA)突變之cybrids中，探討內生性coenzyme Q10含量和合成coenzyme Q10所需之人類PDSS或COQ基因和蛋白對氧化壓力或粒線體功能缺失所產生之反應及其調控。接著，我們建立two-dimensional blue native -polyacrylamide gel electrophoresis (2D BN-PAGE)技術來鑑定人類細胞粒線體中含PDSS和數個COQ蛋白之大分子蛋白複合體，也進一步發掘小鼠細胞和組織之粒線體中類似蛋白複合體的存在。

3. 我們利用含致病性mtDNA突變及缺乏mtDNA之ρ0細胞株來評估以Oxford Nanopore長片段次世代定序技術分析人類粒線體DNA突變及拷貝數的最佳條件。

English

1. Our laboratory has established the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes in human and mouse body fluids and mouse tissues through gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS). These markers are gold-standard markers of lipid peroxidation in vivo and we used them to study the roles of oxidative damage in various human diseases or toxicity. The research topics that have utilized this platform in collaboration with clinicians at Linkou Chang Gung Memorial Hospitals are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, immunosuppressive therapy for paraquat (an herbicide) poisoning, and chelation therapy for lead (Pb) and mercury (Hg) poisoning.

2. We have been investigating the regulation of endogenous coenzyme Q10 levels and human PDSS and COQ genes or proteins essential for biosynthesis of coenzyme Q10 in response to oxidative stress and mitochondrial dysfunction in human cells or cybrids harboring mitochondrial DNA (mtNDA) mutations. We have then established the technique of two-dimensional blue native -polyacrylamide gel electrophoresis (2D BN-PAGE) to identify high-molecular-weight protein complexes PDSS and several COQ proteins in the mitochondria of human cells and to further explore the existence of similar protein complexes in the mitochondria of mouse cells and tissues.

3. We have been evaluating the optimal conditions of analyzing human mtDNA mutations and copy number by Oxford Nanopore long-read next generation sequencing through the use of cybrid cells harboring pathogenic mtDNA mutations and ρ0 cells lacking mtDNA.