研究方向
Research Direction

基礎研究 (Basic Research)

1. Regulation of endogenous coenzyme Q10 (CoQ10) levels and human PDSS or COQ genes and proteins essential for biosynthesis of CoQ10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids.

2. Regulation of endogenous coenzyme Q10 (CoQ10) levels and human PDSS or COQ genes essential for the biosynthesis of coenzyme Q10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids harboring pathogenic mutations of mitochondrial DNA (mtDNA).

3. Characterization of human PDSS and COQ proteins and mouse Pdss and Coq proteins and identification of high-molecular-weight protein complexes in the mitochondria.

臨床及轉譯研究 (Clinical and Translational Research)

1. Establishment of the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes, which are the most specific markers of lipid peroxidation in vivo, in body fluids (CSF, plasma, urine), and tissues by the gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) method for studying the relationship between various human conditions and oxidative damage. The research topics that have been applied this platform in collaboration with clinicians at the Linkou Chang Gung Memorial Hospital are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, paraquat (an herbicide) poisoning, and lead (Pb) poisoning.

2. The investigation on the correlation between the changes related to primary antioxidant enzymes, CoQ10 , and somatic mtDNA mutations and grades or malignancy of human astrocytoma tissues.