Selected Publications

Note: # equal contribution / co-first author

1. Cultivating advanced embryo models through bioengineering mastery

Xufeng Xue, Yue Liu, and Jianping Fu

Nature Reviews Bioengineering, 2024, in press

2. A human pluripotent stem cell-based somitogenesis model using microfluidics

Yue Liu, Yung Su Kim, Xufeng Xue, Norio Kobayashi, Shiyu Sun, Qiong Yang, Olivier Pourquié, and Jianping Fu

Cell Stem Cell, 2024. Accepted

DOI: 10.1101/2023.10.29.564399

3. A patterned human neural tube model using microfluidic gradients

Xufeng Xue, Yung Su Kim, Alfredo-Isaac Ponce-Arias, Richard O'Laughlin, Robin Zhexuan Yan, Norio Kobayashi, Rami Yair Tshuva, Yu-Hwai Tsai, Shiyu Sun, Yi Zheng, Yue Liu, Frederick C.K. Wong, Azim Surani, Jason Spence, Hongjun Song, Guo-Li Ming, Orly Reiner, and Jianping Fu

Nature, vol. 628, 391-399, 2024

DOI: 10.1038/s41586-024-07204-7

4. Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells

Jonathon Muncie, Nadia Ayad, Johnathon Lakins, Xufeng Xue, Jianping Fu, Valerie Weaver

Developmental Cell, vol. 55, pp. 679-694, 2020

DOI: 10.1016/j.devcel.2020.10.015

5. Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a biomimetic neurogenic niche

Yuanyuan Zheng#, Xufeng Xue#, Agnes M. Resto-Irizarry, Zida Li, Yue Shao, Yi Zheng, Gang Zhao, and Jianping Fu

Science Advances, vol. 5, eaax5933, 2019

DOI: 10.1126/sciadv.aax5933

6. Controlled modeling of human epiblast and amnion development using stem cells

Yi Zheng, Xufeng Xue, Yue Shao, Sicong Wang, Sajedeh Nasr Esfahani, Zida Li, Jonathon Muncie, Johnathon N. Lakins, Valerie M. Weaver, Deborah L. Gumucio, and Jianping Fu

Nature, vol. 573, pp. 421-425, 2019

DOI: 10.1038/s41586-019-1535-2

• Commentary by Amander T. Clark, Nature, vol. 573, pp. 350-351, 2019

7. Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells

Xufeng Xue#, Yubing Sun#, Agnes M. Resto-Irizarry, Ye Yuan, Koh Meng Aw Yong, Yi Zheng, Shinuo Weng, Yue Shao, Yimin Chai, Lorenz Studer, and Jianping Fu

Nature Materials, vol. 17, pp. 633-641, 2018

DOI: 10.1038/s41563-018-0082-9

• Commentary by Mukul Tewary and Peter W. Zandstra, Nature Materials, vol. 17, pp. 571-572, 2018

Publications

Google Scholar (Note: # equal contribution / co-first author)

Submitted

1. Self-organized, peri-gastrulation human embryo model with trilaminar embryonic disc-, amnion- and yolk sac-like structures

Shiyu Sun, Yi Zheng, Norio Kobayashi, Yung Su Kim, Xufeng Xue, Yue Liu, Yanhong Xu, Jinglei Zhai, Hongmei Wang, and Jianping Fu. 

Nature, under review

2. Generation of fate patterns via intercellular forces

Hayden Nunley, Xufeng Xue, Jianping Fu, and David K. Lubensky

Physical Review Letters, under review

2024

3. Cultivating advanced embryo models through bioengineering mastery

Xufeng Xue, Yue Liu, and Jianping Fu

Nature Reviews Bioengineering, 2024, in press

4. A human pluripotent stem cell-based somitogenesis model using microfluidics

Yue Liu, Yung Su Kim, Xufeng Xue, Norio Kobayashi, Shiyu Sun, Qiong Yang, Olivier Pourquié, and Jianping Fu

Cell Stem Cell, 2024. Accepted

DOI: 10.1101/2023.10.29.564399

5. Metabolic-glycoengineering-enabled molecularly specific acoustic tweezing cytometry for targeted mechanical stimulation of cell surface sialoglycans

Weiping Li, Jiatong Guo, Eric C Hobson, Xufeng Xue, Qingjiang Li, Jianping Fu, Cheri X Deng, and Zhongwu Guo

Angewandte Chemie, vol. 63, e202401921, 2024

DOI: 10.1002/anie.202401921

6. A patterned human neural tube model using microfluidic gradients

Xufeng Xue, Yung Su Kim, Alfredo-Isaac Ponce-Arias, Richard O'Laughlin, Robin Zhexuan Yan, Norio Kobayashi, Rami Yair Tshuva, Yu-Hwai Tsai, Shiyu Sun, Yi Zheng, Yue Liu, Frederick C.K. Wong, Azim Surani, Jason Spence, Hongjun Song, Guo-Li Ming, Orly Reiner, and Jianping Fu

Nature, vol. 628, 391-399, 2024

DOI: 10.1038/s41586-024-07204-7

7. Derivation of human primordial germ cell-like cells in an embryonic-like culture

Sajedeh Nasr Esfahani, Yi Zheng, Auriana Arabpour, Agnes M. Resto Irizarry, Norio Kobayashi, Xufeng Xue, Yue Shao, Cheng Zhao, Nicole L. Agranonik, Megan Sparrow, Timothy J. Hunt, Jared Faith, Mary Jasmine Lara, Qiu Ya Wu, Sherman Silber, Sophie Petropoulos, Ran Yang, Kenneth R. Chien, Amander T. Clark, and Jianping Fu

Nature Communications, vol. 15, 167, 2024

DOI: 10.1038/s41467-023-43871-2

8. Morphogenesis beyond in vivo

Yue Liu, Xufeng Xue, Shiyu Sun, Norio Kobayashi, Yung Su Kim, Jianping Fu

Nature Reviews Physics, vol. 6, 28-66, 2024

DOI: 10.1038/s42254-023-00669-x

2023

9. Rapid responses of human pluripotent stem cells to cyclic mechanical strains applied to integrin by acoustic tweezing cytometry

Zhaoyi Xu#, Shiying Liu#, Xufeng Xue#, Weiping Li, Jianping Fu, and Cheri X. Deng

Scientific Reports, vol. 12, 18030, 2023

DOI: 10.1038/s41598-023-45397-5

10. Modeling development using microfluidics: Bridging gaps to foster fundamental and translational research

Shiyu Sun, Xufeng Xue, Jianping Fu

Current Opinion in Genetics & Development, vol. 82, 102097, 2023

DOI: 10.1016/j.gde.2023.102097

2022

11. Single-cell analysis of human embryoids reveals lineage diversification roadmaps of early development

Yi Zheng, Robin Zhexuan Yan, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang, Alexander Goedel, Yu Kang, Xufeng Xue, Sajedeh Nasr Esfahani, Yue Liu, Agnes M. Resto Irizarry, Weisheng Wu, Yunxiu Li, Weizhi Ji, Yuyu Niu, Kenneth R. Chien, Tianqing Li, Toshihiro Shioda, and Jianping Fu

Cell Stem Cell, vol. 29, pp. 1402-1419, 2022

DOI: 10.1016/j.stem.2022.08.009

2021

12. Single-cell sequencing to unveil the mystery of embryonic development

Zida Li, Feng Lin, Shue Wang, Xufeng Xue, and Yue Shao

Advanced Biology, 2101151, 2021

DOI: 10.1002/adbi.202101151

13. Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation

Sajedeh Nasr Esfahani, Xufeng Xue, Agnes M. Resto Irizarry, Yue Shao, and Jianping Fu

Nano Today, vol. 41, 101310, 2021

DOI: 10.1016/j.nantod.2021.101310

14. Branching development of early post-implantation human embryonic-like tissues in 3D stem cell culture

Kejie Chen, Yi Zheng, Xufeng Xue, Yue Liu, Anges M. Resto-Irizarry, Huajing Tang, and Jianping Fu

Biomaterials, vol. 275, 120898, 2021

DOI: 10.1016/j.biomaterials.2021.120898

2020

15. Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells

Jonathon Muncie, Nadia Ayad, Johnathon Lakins, Xufeng Xue, Jianping Fu, Valerie Weaver

Developmental Cell, vol. 55, pp. 679-694, 2020

DOI: 10.1016/j.devcel.2020.10.015

16. Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation

Zhenzhen Fan, Xufeng Xue, Jianping Fu, and Cheri X. Deng

Scientific Reports, vol. 10, 18253, 2020

DOI: 10.1038/s41598-020-75347-4a

17. Modeling of human neurulation using bioengineered pluripotent stem cell culture

Xufeng Xue, Ryan P. Wang, and Jianping Fu

Current Opinion in Biomedical Engineering, vol. 13, pp. 127-133, 2020

DOI: 10.1016/j.cobme.2020.02.002

2019

18. Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a biomimetic neurogenic niche

Yuanyuan Zheng#, Xufeng Xue#, Agnes M. Resto-Irizarry, Zida Li, Yue Shao, Yi Zheng, Gang Zhao, and Jianping Fu

Science Advances, vol. 5, eaax5933, 2019

DOI: 10.1126/sciadv.aax5933

19. Controlled modeling of human epiblast and amnion development using stem cells

Yi Zheng, Xufeng Xue, Yue Shao, Sicong Wang, Sajedeh Nasr Esfahani, Zida Li, Jonathon Muncie, Johnathon N. Lakins, Valerie M. Weaver, Deborah L. Gumucio, and Jianping Fu

Nature, vol. 573, pp. 421-425, 2019

DOI: 10.1038/s41586-019-1535-2

• Commentary by Amander T. Clark, Nature, vol. 573, pp. 350-351, 2019

• Highlighted by Nature News, Nature Podcast, National Public Radio (NPR) News, BBC News, MIT Technology Review, and Chemical Engineering News (C&EN)

20. Microengineered human amniotic ectoderm tissue array for high-content developmental phenotyping

Sajedeh Nasr Esfahani#, Yue Shao#, Agnes M Resto-Irizarry, Zida Li, Xufeng Xue, Deborah L Gumucio, and Jianping Fu

Biomaterials, vol. 216, pp. 119244, 2019

DOI: 10.1016/j.biomaterials.2019.119244

2018

21. Biophysical phenotypes and determinants of anterior vs. posterior primitive streak cells derived from human pluripotent stem cells

Feng Lin, Yue Shao, Xufeng Xue, Yi Zheng, Zida Li, Chunyang Xiong, Jianping Fu

Acta Biomaterialia, vol. 86, pp. 125-134, 2018

DOI: 10.1016/j.actbio.2019.01.017

22. Acoustic actuation of integrin-bound microbubbles for mechanical phenotyping during differentiation and morphogenesis of human embryonic stem cells

Xufeng Xue#, Zhenzhen Fan#, Reshani Perera, Sajedeh Nasr Esfahani, Agata A. Exner, Jianping Fu and Cheri X. Deng

Small, vol. 14, 1803137, 2018

DOI: 10.1002/smll.201803137

23. Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells

Xufeng Xue#, Yubing Sun#, Agnes M. Resto-Irizarry, Ye Yuan, Koh Meng Aw Yong, Yi Zheng, Shinuo Weng, Yue Shao, Yimin Chai, Lorenz Studer, and Jianping Fu

Nature Materials, vol. 17, pp. 633-641, 2018

DOI: 10.1038/s41563-018-0082-9

• Commentary by Mukul Tewary and Peter W. Zandstra, Nature Materials, vol. 17, pp. 571-572, 2018

• Highlighted by Michigan News, Michigan Engineering, UMass News, Phys.org, Futurity

24. Acoustic tweezing cytometry induces rapid initiation of human embryonic stem cell differentiation

Tugba Topal#, Xiaowei Hong#, Xufeng Xue, Zhenzhen Fan, Ninad Kanetkar, Joe T. Nguyen, Jianping Fu, Cheri X. Deng, and Paul H. Krebsbach

Scientific Reports, vol. 8, 12977, 2018

DOI: 10.1038/s41598-018-30939-z

25. A systems mechanobiology model to identify biomaterials that support cardiac reprogramming

Yen P. Kong, Ana Y. Rioja, Xufeng Xue, Yubing Sun, Jianping Fu, and Andrew J. Putnam

Biomaterials, vol. 181, pp. 280-292, 2018

DOI: 10.1016/j.biomaterials.2018.07.036

26. Carbon Nanotube Strain Sensor Based Hemoretractometer for Blood Coagulation Testing

Zida Li, Yize Wang, Xufeng Xue, Brendan McCraken, Kevin Ward, and Jianping Fu

ACS Sensors, vol. 3, pp. 670–676, 2018

DOI: 10.1021/acssensors.7b00971

2017

27. Capillary assisted deposition of carbon nanotube film for strain sensing

Zida Li, Xufeng Xue, Feng Lin, Yize Wang, Kevin Ward, and Jianping Fu

Applied Physics Letters, vol. 111, 173105, 2017

DOI: 10.1063/1.5001754

28. Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche

Yue Shao#, Kenichiro Taniguchi#, Katherine Gurdziel, Ryan F. Townshend, Xufeng Xue, Koh Meng Aw Yong, Jianming Sang, Jason R. Spence, Deborah L. Gumucio, and Jianping Fu

Nature materials, vol. 16, pp. 419-425, 2017

DOI: 10.1038/nmat4829

• Commentary by Martin Pera, Nature Materials, vol. 16, pp. 394-395, 2017

29. Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation

Xufeng Xue, Xiaowei Hong, Zida Li, Cheri X. Deng, and Jianping Fu

Biomaterials, vol. 134, pp. 22-30, 2017

DOI: 10.1016/j.biomaterials.2017.04.039

30. Notch signaling in regulating angiogenesis in a 3D biomimetic environment

Yi Zheng#, Shue Wang#, Xufeng Xue, Alan Xu, Wei Liao, Alice Deng, Guohao Dai, Allen P. Liu, and Jianping Fu

Lab on a Chip, vol. 17, pp. 1948-1959, 2017

DOI: 10.1039/c7lc00186j

2016

31. Emerging Roles of YAP/TAZ in Mechanobiology

Yubing Sun, Yue Shao, Xufeng Xue, and Jianping Fu

Molecular and Cellular Mechanobiology. (edited by Shu Chien, Adam J. Engler, and Yingxiao Wang) Springer, 2016

DOI: 10.1007/978-1-4939-5617-3_4

Patent:

1. Microfluidic devices and methods for the development of neural tube-like tissues or neural spheroids

Xufeng Xue, Jianping Fu, Huajing Tang

U.S. Patent application, US-20240060039-A1