Work Packages

The project is divided into 5 work packages (WP): 

WP1: Management and dissemination 

WP2: Rosemary SW preparation and valorization 

WP3: Simulation of GI digestion and fecal fermentation 

WP4: Metabolomic and antioxidant activity analysis 

WP5: Data collection and processing 

In the experimental phase, rosemary waste will first undergo steam distillation to simulate essential oil (EO) production, followed by fermentation with L. plantarum as an valorization process. After GI digestion, the samples will be used for fecal fermentation. For phenolic and metabolite analysis, UHLC-MS/MS will be used, while SCFAs will be quantified by GC-MS. 

As most studies have investigated the antioxidant activity of rosemary extracts in homogeneous systems, the WasteDigest project will investigate their activity in multi-phase system, providing insights into their activity in heterogeneous food matrices. In addition, in vivo antioxidant activity will be evaluated using the yeast S. cerevisiae as a model organism, which provides insight into in vivo system.