Red Dye 40

What effect does Red Dye 40 have on chicken embryo development?

Red dye 40 is a widely used synthetic preservative that is commonly found in a variety of foods, drugs, and cosmetics. Examples include items such as jellybeans, fruit flavored gummies, breakfast cereals, frosting/icing, popsicles, fruit punch, and sports drinks

Hypothesis:

We predict that as concentration of Red Dye 40 increases, abnormalities, mortality, and mass of chicken embryos will also increase.

Procedure:

•We created stock solutions of low and high concentrations of Red Dye 40.

-Low contained 0.1012g/mL of Red Dye 40

-High contained 0.02008g/mL of Red Dye 40

•We injected 0.1 mL of different concentrations of Red Dye 40 into the air sack of chicken eggs that were incubated for seven days. Control eggs were injected with 0.1 mL of saline.

•Eggs were sealed and incubated for an additional 7 days.

•On day 14, we extracted the embryos and measured mass (g), length and width (cm), abnormalities, and mortality.

Data Collected:

→Abnormalities

→Mass of Embryo

→Length and Width of Embryo

→Mortality

Results:

Discussion:

•Injection of Red Dye 40 had no statistically significant results on the developing embryos’ weights

•Injection of Red Dye 40 had no effect on the presence of abnormalities in our developing embryos

•Injection of Red Dye 40 had no effect on the mortality rates of the developing embryos

•There were no statistically significant findings correlated to the embryo’s length and width

Future Direction:

•Future studies should

-Conduct this experiment with a larger sample size

-Use higher concentrations of Red Dye 40 to determine if there would be a statistical difference

-Experiment on different injection sites

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