From our identified candidates, we next then checked if there was homology with other proteins, thus either elucidating the function of the peptide or , if no significant hits are found, by indicating as a novel sequence. A consensus sequence from each chosen module was derived using multiple sequence alignment with ambiguities were left as X's. The conserved region was compared to other proteins in the BLAST database.
Negative Hits--short sequences that showed no significant similarity to other sequences (across all organisms) in the BLAST database. Potential novel sequences to explore further.
Positive Hits--sequences that showed similarity to other proteins. To be used to gather functions.