Techniques and equipment
Techniques used in the laboratory
Animal model of infecitons (use of insect to test infections in Drosophila melanogaster, Galleria mellonella, etc)
Biofilms analysis (flow cell system, calgary, microtitter plates, tubes, analysis by confocal and quantification by COMSTAT software, etc)
Molecular biology (clonning, PCR, Real time PCR, RNAseq, microarray, etc)
Microbiology (bacterial culture, growth, quantification, isolation, identification, methabolic studies, etc)
Gene analysis (transcription and traduction analysis, gene fusion, RNA quantification (northern, RealTime PCR, RNAseq, microarrays, etc)
DNA and RNA analysis (isolation, purification, quantification, electrophoresis, southern and norther blot, etc)
Protein analysis (overproduction, purification, electrophoresis, characterization, enzyme analysis, etc)
EMSA (electrophoretic mobility shift assays - analysis protein and DNA interactions)
Microscopy analysis (own confocal microscope and fluorescent microscope)
Bacterial genetics (transduction, transposon analysis, conjugation, chromosomal mutants, etc)
Cell biology (cell growth and infection with cells, etc).
Anaerobiosis analysis (single channer optic oxygen meter with microsensors, anaerotrans system, etc)
Zeiss LSM 800 Confocal Laser Scanning Microscope.
Nikon Inverted Fluorescent microscope ECLIPSE Ti-S/L100.
Cell culture facilities for microbial infections.
The Spark® multimode microplate reader (TECAN)- anaerobic control / humidity cassete.
Biological safety cabinet (class II).
Characterization of nanoparticles/biomaterial antibacterial activity.
Drosophila melanogaster and Galleria mellonella as a model host for bacterial infections.
Continuous flow system model for bacterial biofilm development. Diverse methods for biofilm detection and analysis (Calgary, Drip Flow, flow cells, microfluidics, tube, etc).
Single Channel Fiber-Optic Oxygen Meter with microsensor.
Gradient thermocyclers (PCR).
Molecular biology facilities, electroporation, etc.
Protein and DNA electrophoresis.
Bacterial expression systems for heterologous protein production.
Protein purification systems (FPLC; Biologic DuoFlow System; Bio-Rad).
Technology of microbial culture facilities (microbiological cabin, incubators statics and shakers).
Pressure microinjection system.