Techniques and equipment

Techniques used in the laboratory

  • Molecular biology (clonning, PCR, Real time PCR, RNAseq, microarray, etc)
  • Microbiology (bacterial culture, growth, quantification, isolation, identification, methabolic studies, etc)
  • Gene analysis (transcription and traduction analysis, gene fusion, RNA quantification (northern, RealTime PCR, RNAseq, microarrays, etc)
  • DNA and RNA analysis (isolation, purification, quantification, electrophoresis, southern and norther blot, etc)
  • Protein analysis (overproduction, purification, electrophoresis, characterization, enzyme analysis, etc)
  • EMSA (electrophoretic mobility shift assays - analysis protein and DNA interactions)
  • Microscopy analysis (own confocal microscope and fluorescent microscope)
  • Bacterial genetics (transduction, transposon analysis, conjugation, chromosomal mutants, etc)
  • Animal model of infecitons (use of insect to test infections in Drosophila melanogaster, Galleria mellonella, etc)
  • Biofilms analysis (flow cell system, calgary, microtitter plates, tubes, analysis by confocal and quantification by COMSTAT software, etc)
  • Cell biology (cell growth and infection with cells, etc).
  • Anaerobiosis analysis (single channer optic oxygen meter with microsensors, anaerotrans system, etc)

Equipment

  • Zeiss LSM 800 Confocal Laser Scanning Microscope
  • Nikon Inverted Fluorescent microscope ECLIPSE Ti-S/L100
  • Cell culture facilities for microbial infections
  • The SparkĀ® multimode microplate reader (TECAN)- anaerobic control / humidity cassete.
  • Biological safety cabinet (class II)
  • Characterization of nanoparticles/biomaterial antibacterial activity
  • Drosophila melanogaster and Galleria mellonella as a model host for bacterial infections
  • Continuous flow system model for bacterial biofilm development
  • Single Channel Fiber-Optic Oxygen Meter with microsensor
  • Gradient thermocycler (PCR)
  • Molecular biology facilities
  • Protein and DNA electrophoresis
  • Bacterial expression systems for heterologous protein production
  • Protein purification systems (FPLC; Biologic DuoFlow System; Bio-Rad)
  • Technology of microbial culture facilities
  • Pressure microinjection system