Techniques and equipment

Techniques used in the laboratory

  • Animal model of infecitons (use of insect to test infections in Drosophila melanogaster, Galleria mellonella, etc)

  • Biofilms analysis (flow cell system, calgary, microtitter plates, tubes, analysis by confocal and quantification by COMSTAT software, etc)

  • Molecular biology (clonning, PCR, Real time PCR, RNAseq, microarray, etc)

  • Microbiology (bacterial culture, growth, quantification, isolation, identification, methabolic studies, etc)

  • Gene analysis (transcription and traduction analysis, gene fusion, RNA quantification (northern, RealTime PCR, RNAseq, microarrays, etc)

  • DNA and RNA analysis (isolation, purification, quantification, electrophoresis, southern and norther blot, etc)

  • Protein analysis (overproduction, purification, electrophoresis, characterization, enzyme analysis, etc)

  • EMSA (electrophoretic mobility shift assays - analysis protein and DNA interactions)

  • Microscopy analysis (own confocal microscope and fluorescent microscope)

  • Bacterial genetics (transduction, transposon analysis, conjugation, chromosomal mutants, etc)

  • Cell biology (cell growth and infection with cells, etc).

  • Anaerobiosis analysis (single channer optic oxygen meter with microsensors, anaerotrans system, etc)

Equipment

  • Zeiss LSM 800 Confocal Laser Scanning Microscope.

  • Nikon Inverted Fluorescent microscope ECLIPSE Ti-S/L100.

  • Cell culture facilities for microbial infections.

  • The Spark® multimode microplate reader (TECAN)- anaerobic control / humidity cassete.

  • Biological safety cabinet (class II).

  • Characterization of nanoparticles/biomaterial antibacterial activity.

  • Drosophila melanogaster and Galleria mellonella as a model host for bacterial infections.

  • Continuous flow system model for bacterial biofilm development. Diverse methods for biofilm detection and analysis (Calgary, Drip Flow, flow cells, microfluidics, tube, etc).

  • Single Channel Fiber-Optic Oxygen Meter with microsensor.

  • Gradient thermocyclers (PCR).

  • Molecular biology facilities, electroporation, etc.

  • Protein and DNA electrophoresis.

  • Bacterial expression systems for heterologous protein production.

  • Protein purification systems (FPLC; Biologic DuoFlow System; Bio-Rad).

  • Technology of microbial culture facilities (microbiological cabin, incubators statics and shakers).

  • Pressure microinjection system.