I started this project itching to get hands-on and "show myself" why, and when, steady-state flux balance analysis (FBA) is not enough in metabolic modelling while following Dr. Bernhard Palsson and Dr. Uri Alon's excellent courses, Systems Biology: Constraint-Based Reconstruction and Analysis and Systems Biology.

So I looked for a simple, well-understood case study I could begin from the data of and peek ahead at the "answer" as I vie. Searching for some kind of a "classic application" of kinetic metabolic modelling, I stumbled upon "the original dynamic flux balance analysis (dFBA) paper" by Mahadevan et. al. that proposed the dFBA approach as an algorithm that unlike classic FBA, overcomes FBA's shortcomings on predicting E. coli's diauxic shift when grown on glucose alone.