Skills

Experimental Skills:

  • Molecular Biology: RNA and DNA extraction, cDNA cloning, SDS-PAGE, Gel electrophoresis, rtPCR, qPCR, Reverse transcription, Western blotting, Cell survival assay, Gene expression assay, Yeast surface display, mammalian cell preservation, Serial Cloner, Genious.

  • Biophysics and Proteomics: Expression and purification of recombinant protein in E. coli, yeast, mammalian cell culture methods, Functional protein purification to technological application, Site-specific mutation, Isotopic labelling of proteins, Protein crystallization, Biochemical and biophysical characterization of protein, Affinity chromatography, Ion exchange chromatography, Size exclusion chromatography, PyMOL, HEX, EasyModeller, COOT.

  • Chemistry: Chemical kinetics, Chemical synthesis, TLC, HPLC, Column chromatography, Rotary vapor evaporator, Schlenk line.

  • Instruments: Scanning Electron Microscope (SEM) (JSM 6490 LA, JEOL), E-Graph (ATTA Bio-instrument, Azure C400), CD spectrophotometer (JASCO J-725), BIORAD Biologic Duoflow, Photomicroscopes equipped with temperature controlling unit, UV-visible spectrophotometer with digital data processing facilities, ABLE Jar Fermenter (BMJ-PI), Flow cytometer (Attune NXT), Inverted microscope (Leica DFC420 C), High-performance Liquid Chromatography (HPLC), IVIS Spectrum imager.

Cells Worked With:

  • Cancer cells: GBM6, GBM10, GBM12, GBM22, HF2303, HF3253, HF2427, HF2353, HF3016, U-87, U251, MBAMD-231, MBAMD-157, MBAMD-468, SUM149, SUM159, MIA, HN26 and HN48.

  • Recombinant protein expression and molecular biological experiments: HEK293, BL21(DE3), JM109, NEB stable, DH5αZ1, RJY100.

Others skills:

  • Trainings on Laboratory Safety, Biohazard, Bloodborne Pathogens, Biosafety and Environmental protection.

  • English (academic), Bangla (Native), Hindi (Spoken), Japanese (spoken) & Arabic (reading)

  • Lead most of the departmental programs and cultural events organized by Department of Chemistry, University of Dhaka (from April 2004-December 2010).

  • Volunteer in 2nd Ice Binding Protein (IBP) Conference held in Sapporo, Japan.

  • Editor and designer of the Souvenir, Abstracts and Programme of Bangladesh Chemical Congress 2008 and First Humboldt Kollege International Conference in Dhaka, Bangladesh.