Skills
Experimental Skills:
Molecular Biology: RNA and DNA extraction, cDNA cloning, SDS-PAGE, Gel electrophoresis, rtPCR, qPCR, Reverse transcription, Western blotting, Cell survival assay, Gene expression assay, Yeast surface display, mammalian cell preservation, Serial Cloner, Genious.
Biophysics and Proteomics: Expression and purification of recombinant protein in E. coli, yeast, mammalian cell culture methods, Functional protein purification to technological application, Site-specific mutation, Isotopic labelling of proteins, Protein crystallization, Biochemical and biophysical characterization of protein, Affinity chromatography, Ion exchange chromatography, Size exclusion chromatography, PyMOL, HEX, EasyModeller, COOT.
Chemistry: Chemical kinetics, Chemical synthesis, TLC, HPLC, Column chromatography, Rotary vapor evaporator, Schlenk line.
Instruments: Scanning Electron Microscope (SEM) (JSM 6490 LA, JEOL), E-Graph (ATTA Bio-instrument, Azure C400), CD spectrophotometer (JASCO J-725), BIORAD Biologic Duoflow, Photomicroscopes equipped with temperature controlling unit, UV-visible spectrophotometer with digital data processing facilities, ABLE Jar Fermenter (BMJ-PI), Flow cytometer (Attune NXT), Inverted microscope (Leica DFC420 C), High-performance Liquid Chromatography (HPLC), IVIS Spectrum imager.
Cells Worked With:
Cancer cells: GBM6, GBM10, GBM12, GBM22, HF2303, HF3253, HF2427, HF2353, HF3016, U-87, U251, MBAMD-231, MBAMD-157, MBAMD-468, SUM149, SUM159, MIA, HN26 and HN48.
Recombinant protein expression and molecular biological experiments: HEK293, BL21(DE3), JM109, NEB stable, DH5αZ1, RJY100.
Others skills:
Trainings on Laboratory Safety, Biohazard, Bloodborne Pathogens, Biosafety and Environmental protection.
English (academic), Bangla (Native), Hindi (Spoken), Japanese (spoken) & Arabic (reading)
Lead most of the departmental programs and cultural events organized by Department of Chemistry, University of Dhaka (from April 2004-December 2010).
Volunteer in 2nd Ice Binding Protein (IBP) Conference held in Sapporo, Japan.
Editor and designer of the Souvenir, Abstracts and Programme of Bangladesh Chemical Congress 2008 and First Humboldt Kollege International Conference in Dhaka, Bangladesh.