This single-author preprint revisits local timing differences among neighboring sarcomeres during hyperthermal sarcomeric oscillations and asks whether that local nonuniformity is random or organized by a shared rephasing order across cells. A topology-based circular coordinate and cross-cell alignment yield a shared rephasing compass. The clearest primary readout remains mismatch-pocket placement along the observed five-sarcomere chain. Version 2 extends this picture by adding a reduced-geometry view of how the same minimal update is internally distributed within the chain, termed internal packaging.
Key Points
A topology-based circular coordinate plus cross-cell alignment yields a shared rephasing compass across cells.
The strongest and most robust primary readout is mismatch-pocket placement / edge bias along the observed five-sarcomere chain.
Reduced-geometry analysis adds a second linked phenotype, internal packaging, which asks how the same minimal update is internally distributed within the chain.
Across common matched groups, the extended reduced-geometry family shows broader internal span in 8 of 9 groups (median span difference +0.423; paired Wilcoxon P = 0.027).
Edge bias itself does not shift consistently across those matched comparisons, indicating that internal packaging adds information beyond simple edge preference.
Beat timing weakens to a secondary result, and signed strain weakens further as an explanatory descriptor.
Together, the results support a structured mesoscale view of local HSO nonuniformity across linked readouts rather than random local disorder.
Seine A. Shintani. Structured local mismatch placement and internal packaging in cardiomyocytes during hyperthermal sarcomeric oscillations. bioRxiv (Preprint, 2026).
DOI: 10.64898/2026.03.26.714639
Keywords: Hyperthermal Sarcomeric Oscillations (HSOs), Cardiomyocyte, Rephasing Compass, Local Mismatch Placement, Internal Packaging, Mesoscale Coordination